Disposable collection kit for rapid and reliable collection of saliva

American Journal of Human Biology : the Official Journal of the Human Biology Council
Masaki YamaguchiVivek Shetty

Abstract

To describe and evaluate disposable saliva collection kit for rapid, reliable, and reproducible collection of saliva samples. The saliva collection kit comprised of a saliva absorbent swab and an extractor unit was used to retrieve whole saliva samples from 10 subjects. The accuracy and precision of the extracted volumes (3, 10, and 30 μl) were compared to similar volumes drawn from control samples obtained by passive drool. Additionally, the impact of kit collection method on subsequent immunoassay results was verified by assessing salivary cortisol levels in the samples and comparing them to controls. The recovered volumes for the whole saliva samples were 3.85 ± 0.28, 10.79 ± 0.95, and 31.18 ± 1.72 μl, respectively (CV = 8.76%) and 2.91 ± 0.19, 9.75 ± 0.43, and 29.64 ± 0.91 μl, respectively, (CV = 6.36%) for the controls. There was a close correspondence between the salivary cortisol levels from the saliva samples obtained by the collection kit and the controls (R(2)  > 0.96). The disposable saliva collection kit allows accurate and repeatable collection of fixed amounts of whole saliva and does not interfere with subsequent measurements of salivary cortisol. The simple collection process, lack of elaborate specimen recovery...Continue Reading

References

Jan 1, 1994·Psychoneuroendocrinology·C Kirschbaum, D H Hellhammer
Sep 20, 1993·Annals of the New York Academy of Sciences·I D Mandel
Mar 7, 2007·Occupational and Environmental Medicine·David Soo-Quee Koh, Gerald Choon-Huat Koh
Dec 20, 2008·Psychoneuroendocrinology·Dirk H HellhammerBrigitte M Kudielka
May 18, 2012·Biological Research for Nursing·Douglas A GrangerLynette Lau Schumann
Sep 14, 2012·Applied Biochemistry and Biotechnology·Aihua ZhangXijun Wang
Nov 12, 2013·Biosensors & Bioelectronics·Ajeet KaushikShekhar Bhansali

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Methods Mentioned

BETA
ELISA
biosensor

Software Mentioned

Statistical Package for the Social Sciences ( SPSS )

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