PMID: 11932961Apr 5, 2002Paper

Disposition and receptor-site binding of (125)I-EGF after topical administration to skin wounds

Biopharmaceutics & Drug Disposition
P A PratsE Fernández-Sánchez

Abstract

The rhEGF topical delivery systems have been hindered by a number of shortcomings which have led to the search of new development strategies. In this study we report the evaluation of cumulative profiles of 10, 5 and 1 microg/ml solutions of (125)I-rhEGF, in a rat full-thickness skin wound model, as well as the drug-induced modulation in the expression of the EGF receptor after lesion. The tissue-associated radioactivity, expressed as the percentage of the dose administered per grams of tissue (%D/g), peaks at 2 h after administration of all doses. (125)I-rhEGF degraded species were detected chromatographically, but no diffusion of the peptide to the surrounding skin was documented. Despite the dose, the EGF receptor expression was increased within 2 h after wounding, followed by a slow decline up to 12 h below baseline. Twelve hours after punch, differences were evident between all treated groups and control. These results demonstrate that (125)I-rhEGF saline solutions are rapidly cleared from application sites, probably by protease-driven cleavage and receptor-mediated endocytosis. Finally, we must be aware that the results herein discussed should be taken into account during the drug delivery system design in order to guaran...Continue Reading

References

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Citations

May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·M L RibeiroA M Franchi
Sep 14, 2011·International Wound Journal·Jorge Berlanga-Acosta
Jan 18, 2005·International Journal of Pharmaceutics·K H MatthewsG M Eccleston
Oct 4, 2016·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Rahman ŞenocakOrhan Kozak
Sep 15, 2017·BioMed Research International·Jorge Berlanga-AcostaGerardo Guillén-Nieto

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