Disposition of triazolam in the rat by brain microdialysis and semi-micro column high-performance liquid chromatography with UV absorbance detection

Biomedical Chromatography : BMC
Kenichiro NakashimaMihoko N Nakashima

Abstract

A semi-micro column high-performance liquid chromatography with ultraviolet detection for the determination of triazolam is described. The method was applied to determine plasma and brain microdialysate concentrations of triazolam after single intravenous bolus of 2.5 mg/kg to rat. The separation was achieved on a 250 x 1.5 mm i.d. C(18) column and the column effluent was monitored at 222 nm. The detection limits at a signal-to-noise ratio of 3 obtained using spiked plasma and artificial cerebrospinal fluid were 2.1 and 0.7 ng/mL, respectively. The intra- and inter-day reproducibility of the present method were satisfactory with the highest relative standard deviation of 9.1 (n > or = 5). The present method was successfully applied to study the disposition of triazolam in rat (n = 5) by analyzing plasma and brain microdialysate samples.

References

Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Jan 1, 1981·Clinical Pharmacology and Therapeutics·F S EbertsR W Vliek
Nov 1, 1981·Journal of Pharmacobio-dynamics·K YamaokaT Uno
Sep 1, 1996·Journal of Pharmaceutical Sciences·J GaudreaultG M Pollack
Mar 1, 1997·Pharmaceutical Research·W F Elmquist, R J Sawchuk
Jul 29, 1999·Biomedical Chromatography : BMC·C S Chaurasia
Oct 12, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·K Heinig, J Henion

❮ Previous
Next ❯

Citations

Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tung-Hu Tsai
Apr 29, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jin-ping QiaoZong-ru Guo
Dec 18, 2002·Journal of Pharmaceutical and Biomedical Analysis·Kenichiro NakashimaMihoko N Nakashima
Oct 6, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kenichiro Nakashima
Jul 1, 2009·Bioanalysis·Victoria F SamanidouIoannis N Papadoyannis
Dec 3, 2005·Journal of Pharmaceutical and Biomedical Analysis·Kenichiro NakashimaMitsuhiro Wada
Nov 21, 2009·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·T KudoK Sugiyama
Feb 19, 2010·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·T KudoK Sugiyama
Mar 31, 2021·Biomedical Chromatography : BMC·Noha M El ZaharMichael G Bartlett

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.