PMID: 8943148Nov 12, 1996Paper

Disruption of a novel open reading frame of Plasmodium falciparum chromosome 9 by subtelomeric and internal deletions can lead to loss or maintenance of cytoadherence

Molecular and Biochemical Parasitology
P F BourkeD J Kemp

Abstract

Isolates of Plasmodium falciparum commonly undergo a large subtelomeric deletion of the right end of chromosome 9 during in vitro cultivation, accompanied by loss of ability to cytoadhere to melanoma cells and greatly lowered gametocyte production. ItG2, an isolate in which cytoadherence is stable, has undergone a subtelomeric deletion of intermediate length on chromosome 9. We show here that the deletions in all non-cytoadherent clones examined have breakpoints within or delete a novel open reading frame (the breakpoint open reading frame, BPORF) that is a unique sequence in the genome. Surprisingly, in ItG2 BPORF has been removed by an additional 15 kb internal deletion in chromosome 9. These results suggest mechanisms to account for the observed frequent deletion of the right arm of chromosome 9 and for the known stability of cytoadherence in ItG2. However, we were unable to detect var genes in this region of the ItG2 genome. We conclude that the product of a novel gene distinct from the var family is implicated in cytoadherence.

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Citations

Sep 10, 1999·International Journal for Parasitology·D C HoltK R Trenholme
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·J C DujardinD Le Ray
Dec 12, 2001·Molecular and Biochemical Parasitology·D C HoltD J Kemp
Jan 8, 1999·Molecular and Biochemical Parasitology·D C HoltD J Kemp
Aug 22, 2000·Parasitology Today·A Craig
May 24, 2003·Trends in Parasitology·Michelle L GattonQin Cheng
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S GuerboujJ C Dujardin
Apr 17, 2009·Nature Reviews. Microbiology·Alexander G MaierLeann Tilley
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·K R TrenholmeD J Kemp
Nov 10, 2009·PLoS Pathogens·Margaret J MackinnonZbynek Bozdech
Sep 22, 2005·Molecular and Biochemical Parasitology·Céline Karine CarretAlasdair Ivens
Jul 25, 2000·Memórias do Instituto Oswaldo Cruz·J C DujardinD Le Ray
Oct 16, 2004·Molecular and Biochemical Parasitology·Paula L HawthorneDonald L Gardiner

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