PMID: 8970150Dec 1, 1996Paper

Disruption of actin filaments increases the activity of sodium-conducting channels in human myeloid leukemia cells

Molecular Biology of the Cell
Yuri A NegulyaevA V Maximov

Abstract

With the use of the patch clamp technique, the role of cytoskeleton in the regulation of ion channels in plasma membrane of leukemic K562 cells was examined. Single-channel measurements have indicated that disruption of actin filaments with cytochalasin D (CD) resulted in a considerable increase of the activity of non-voltage-gated sodium-permeable channels of 12 pS unitary conductance. Background activity of these channels was low; open probability (po) did not exceed 0.01-0.02. After CD, po grew at least 10-20 times. Cell-attached and whole-cell recordings showed that activation of sodium channels was elicited within 1-3 min after the addition of 10-20 micrograms/ml CD to the bath extracellular solution or in the presence of 5 micrograms/ml CD in the intracellular pipette solution. Preincubation of K562 cells with CD during 1 h also increased drastically the activity of 12 pS sodium channels. Whole-cell measurements confirmed that CD-activated channels were permeable to monovalent cations (preferentially to Na+ and Li+), but not to bivalent cations (Ca2+, Ba2+). Colchicine (1 microM), which affect microtubules, did not alter background channel activity. Our data indicate that actin filaments organization plays an important ro...Continue Reading

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Citations

Apr 11, 2003·Molecular Biology of the Cell·Ekaterina V ShumilinaSofia Yu Khaitlina
Jul 5, 2013·Doklady. Biochemistry and Biophysics·V I Chubinskiy-NadezhdinE A Morachevskaya
Jun 21, 2014·Nanoscale Research Letters·Irina V OgnevaLudmila B Buravkova
May 17, 2002·The Journal of Physiology·Alexandr V Staruschenko, Elena A Vedernikova
Aug 11, 2007·The New Phytologist·Enid A C MacRobbie, Smita Kurup
Apr 11, 2015·Biochemical and Biophysical Research Communications·Anastasia V SudarikovaYuri A Negulyaev
Apr 22, 2005·Biochimica Et Biophysica Acta·Alexander StaruschenkoElena A Morachevskaya
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Jul 28, 2007·Tsitologiia·S Iu Khaĭtlina
Sep 12, 2018·Journal of Cellular Biochemistry·Anastasia V SudarikovaVladislav I Chubinskiy-Nadezhdin
Feb 8, 2003·Hypertension·Stuart A DunnTrevor H Thomas
Oct 6, 2009·Tsitologiia·A V SudarikovaE A Morachevskaia
Sep 28, 2012·American Journal of Physiology. Cell Physiology·Evi SchmidEkaterina Shumilina
Oct 4, 2000·The Journal of Biological Chemistry·Y A NegulyaevE A Vedernikova
Feb 10, 1999·The American Journal of Physiology·X WanC E Morris
Oct 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Zhuren WangDavid Fedida

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