Disruption of BCAA metabolism in mice impairs exercise metabolism and endurance.

Journal of Applied Physiology
Pengxiang SheChristopher J Lynch

Abstract

Exercise enhances branched-chain amino acid (BCAA) catabolism, and BCAA supplementation influences exercise metabolism. However, it remains controversial whether BCAA supplementation improves exercise endurance, and unknown whether the exercise endurance effect of BCAA supplementation requires catabolism of these amino acids. Therefore, we examined exercise capacity and intermediary metabolism in skeletal muscle of knockout (KO) mice of mitochondrial branched-chain aminotransferase (BCATm), which catalyzes the first step of BCAA catabolism. We found that BCATm KO mice were exercise intolerant with markedly decreased endurance to exhaustion. Their plasma lactate and lactate-to-pyruvate ratio in skeletal muscle during exercise and lactate release from hindlimb perfused with high concentrations of insulin and glucose were significantly higher in KO than wild-type (WT) mice. Plasma and muscle ammonia concentrations were also markedly higher in KO than WT mice during a brief bout of exercise. BCATm KO mice exhibited 43-79% declines in the muscle concentration of alanine, glutamine, aspartate, and glutamate at rest and during exercise. In response to exercise, the increments in muscle malate and alpha-ketoglutarate were greater in KO...Continue Reading

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Citations

Aug 26, 2010·The Journal of Biological Chemistry·Yingsheng ZhouPengxiang She
Jul 23, 2013·Exercise and Sport Sciences Reviews·Heikki KainulainenUrho M Kujala
Apr 21, 2011·American Journal of Physiology. Endocrinology and Metabolism·Pengxiang SheChristopher J Lynch
Jan 21, 2015·Biochemical and Biophysical Research Communications·Tomoki SatoShinji Miura
Jun 27, 2013·Molecular Genetics and Metabolism·Heather A ZimmermanChristopher J Lynch
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May 8, 2015·Journal of Nutrition and Metabolism·Paul J ArcieroEmery Ward
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Feb 9, 2017·Nature Medicine·Deborah M Muoio
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Jan 31, 2015·Molecular Endocrinology·Joel S BurrillDavid A Bernlohr
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Sep 10, 2015·Physiological Genomics·Christopher J LynchYuka Imamura Kawasawa
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Jun 3, 2021·International Journal of Environmental Research and Public Health·Abdullah F AlghannamMaha H Alhussain
Aug 7, 2021·Frontiers in Physiology·Gagandeep MannOlasunkanmi A J Adegoke
Sep 21, 2021·Critical Reviews in Food Science and Nutrition·Elżbieta SupruniukAdrian Chabowski

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