Disruption of bidirectional oocyte-cumulus paracrine signaling during in vitro maturation reduces subsequent mouse oocyte developmental competence.

Biology of Reproduction
Christine X YeoMichelle Lane

Abstract

Oocyte-cumulus cell bidirectional communication is essential for normal development of the oocyte and cumulus cells (CCs) within the follicle. We showed recently that addition of recombinant growth differentiation factor 9 (GDF9), which signals through the SMAD2/3 pathway, during mouse oocyte in vitro maturation (IVM) increased fetal viability. This study thus aimed to observe the effects of disrupting oocyte-CC bidirectional communication during IVM on oocyte developmental competence and fetal outcomes. Cumulus-oocyte complexes (COCs) from equine chorionic gonadotropin-primed prepubertal (CBA/C57BL6) mice were cultured with or without 50 mIU/ml follicle-stimulating hormone (FSH) and 10 ng/ml epidermal growth factor (EGF) or 4 muM SMAD2/3 inhibitor SB-431542. Cumulus expansion and first polar body extrusion were then assessed, or COCs were fertilized and stained to evaluate sperm entry or cultured to the blastocyst stage. Embryo development and blastocyst quality were assessed, and Day 4.5 blastocysts were transferred to pseudopregnant recipients to analyze fetal outcomes. SMAD2/3 inhibition or FSH/EGF absence during IVM resulted in decreased cumulus expansion. First polar body extrusion and sperm entry were decreased in the ab...Continue Reading

References

Mar 1, 1990·Biology of Reproduction·C F Fagbohun, S M Downs
Feb 1, 1990·Molecular Reproduction and Development·J J van de SandtJ J Eppig
May 1, 1985·The Journal of Experimental Zoology·H J Leese, A M Barton
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J D BiggersR P Donahue
Nov 1, 1968·Journal of Reproduction and Fertility·R P Donahue, S Stern
Sep 1, 1984·Journal of Reproduction and Fertility·H J Leese, A M Barton
May 1, 1983·Biology of Reproduction·R Colonna, F Mangia
Mar 1, 1997·Molecular Reproduction and Development·A G ByskovX Guoliang
Jun 24, 1999·Molecular Endocrinology·J A ElvinM M Matzuk
Jun 28, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·D F AlbertiniM J Carabatsos
Dec 6, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·J J Eppig
Jan 5, 2002·Molecular Reproduction and Development·Marziel KamjooSusan J Kimber
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·John J EppigFrank L Pendola
Jul 24, 2002·Biology of Reproduction·Ursula A VittAaron J W Hsueh
Feb 8, 2003·The Journal of Clinical Endocrinology and Metabolism·Noora Kaivo-OjaOlli Ritvos
Jun 16, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Franchesca D Houghton, Henry J Leese
Apr 12, 2005·Molecular Reproduction and Development·Ali AtefSirard Marc-André
Aug 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Atef Ali, Marc-André Sirard
Jul 21, 2006·Developmental Biology·Tamer S HusseinRobert B Gilchrist
Aug 24, 2006·Journal of Cell Science·Robert B GilchristDavid G Mottershead
Oct 21, 2006·Animal Reproduction Science·Hakan SagirkayaErdogan Memili
Nov 15, 2006·Animal Reproduction Science·C E FarinS Kennedy-Stoskopf
Dec 29, 2006·Biology of Reproduction·Rebecca A DragovicRobert B Gilchrist

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Citations

Jul 28, 2011·Zygote : the Biology of Gametes and Early Embryos·Ma Ninia L GomezByeong Chun Lee
Mar 30, 2011·Human Reproduction Update·Maurizio ZuccottiSilvia Garagna
May 4, 2010·Molecular Human Reproduction·Zhongwei Huang, Dagan Wells
Jun 6, 2012·TheScientificWorldJournal·Gustavo Adolfo PalmaFred Sinowatz
Nov 1, 2013·Journal of Assisted Reproduction and Genetics·Maurício Machaim FrancoMargot Alves Nunes Dode
Aug 31, 2014·Journal of Assisted Reproduction and Genetics·Li-Na WeiXiao-Yan Liang
Jun 15, 2013·Theriogenology·Sh Rouhollahi VarnosfaderaniM H Nasr-Esfahani
Aug 22, 2014·Biology of Reproduction·Angus D MacaulayClaude Robert
Oct 10, 2014·Biology of Reproduction·Francesca LolicatoBarend M Gadella
Feb 18, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bettina P MihalasJessie M Sutherland
Jun 9, 2020·Biology of Reproduction·Zeinab DehghanMohammad Salehi
Nov 21, 2015·Biology of Reproduction·Angus D MacaulayClaude Robert
Dec 19, 2020·Molecular and Cellular Endocrinology·Fang-Ju WuChing-Wei Luo

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