Disruption of both chloroplastic and cytosolic FBPase genes results in a dwarf phenotype and important starch and metabolite changes in Arabidopsis thaliana

Journal of Experimental Botany
José A Rojas-GonzálezMariam Sahrawy

Abstract

In this study, evidence is provided for the role of fructose-1,6-bisphosphatases (FBPases) in plant development and carbohydrate synthesis and distribution by analysing two Arabidopsis thaliana T-DNA knockout mutant lines, cyfbp and cfbp1, and one double mutant cyfbp cfbp1 which affect each FBPase isoform, cytosolic and chloroplastic, respectively. cyFBP is involved in sucrose synthesis, whilst cFBP1 is a key enzyme in the Calvin-Benson cycle. In addition to the smaller rosette size and lower rate of photosynthesis, the lack of cFBP1 in the mutants cfbp1 and cyfbp cfbp1 leads to a lower content of soluble sugars, less starch accumulation, and a greater superoxide dismutase (SOD) activity. The mutants also had some developmental alterations, including stomatal opening defects and increased numbers of root vascular layers. Complementation also confirmed that the mutant phenotypes were caused by disruption of the cFBP1 gene. cyfbp mutant plants without cyFBP showed a higher starch content in the chloroplasts, but this did not greatly affect the phenotype. Notably, the sucrose content in cyfbp was close to that found in the wild type. The cyfbp cfbp1 double mutant displayed features of both parental lines but had the cfbp1 phenotyp...Continue Reading

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Feb 24, 2010·Journal of Experimental Botany·Iván Iglesias-BaenaJuan-José Lázaro
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Citations

Jan 27, 2016·Plant Science : an International Journal of Experimental Plant Biology·Javier CórdobaPilar Pérez
Jul 28, 2015·Bioscience, Biotechnology, and Biochemistry·Takahisa OgawaShigeru Shigeoka
Apr 4, 2017·The Plant Journal : for Cell and Molecular Biology·Marta BjornsonKatayoon Dehesh
Feb 18, 2019·Journal of Experimental Botany·Andrew J SimkinChristine A Raines
Mar 21, 2019·International Journal of Molecular Sciences·Quan WangXinguo Li
Sep 23, 2021·Annals of Botany·Laura Kathrine PerbyAngelika Mustroph

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Methods Mentioned

BETA
transgenic
genotyping
transmission electron microscopy
light microscopy
fluorescence spectroscopy
PCR

Software Mentioned

Adobe Photoshop
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Photosyn Assistant
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