Disruption of deiodinase type 2 in zebrafish disturbs male and female reproduction

The Journal of Endocrinology
Anne M HoubrechtsVeerle M Darras

Abstract

Thyroid hormones are crucial mediators of many aspects of vertebrate life, including reproduction. The key player is the biologically active 3,5,3'-triiodothyronine (T3), whose local bio-availability is strictly regulated by deiodinase enzymes. Deiodinase type 2 (Dio2) is present in many tissues and is the main enzyme for local T3 production. To unravel its role in different physiological processes, we generated a mutant zebrafish line, completely lacking Dio2 activity. Here we focus on the reproductive phenotype studied at the level of offspring production, gametogenesis, functioning of the hypothalamic-pituitary-gonadal axis and sex steroid production. Homozygous Dio2-deficient zebrafish were hypothyroid, displayed a delay in sexual maturity, and the duration of their reproductive period was substantially shortened. Fecundity and fertilization were also severely reduced. Gamete counts pointed to a delay in oogenesis at onset of sexual maturity and later on to an accumulation of oocytes in mutant ovaries due to inhibition of ovulation. Analysis of spermatogenesis showed a strongly decreased number of spermatogonia A at onset of sexual maturity. Investigation of the hypothalamic-pituitary-gonadal axis revealed that dysregulatio...Continue Reading

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Citations

Jun 5, 2020·Journal of Clinical Medicine·Marco ColellaConcetta Ambrosino
Sep 11, 2019·Endocrinology·Anne M HoubrechtsVeerle M Darras
Nov 27, 2020·Frontiers in Endocrinology·Cole K Deal, Helene Volkoff
Mar 16, 2021·Endocrinology·Samuel C RussoAntonio C Bianco

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