Disruption of follistatin by RNAi increases apoptosis, arrests S-phase of cell cycle and decreases estradiol production in bovine granulosa cells

Animal Reproduction Science
Zhenlu ChongLiguo Yang

Abstract

Follistatin (FST), a local regulator of gonadal functions is a powerful inhibitor of follicle stimulating hormone (FSH) secretion. In the present study, the expression of FST was partially silenced at both transcriptional and translational levels by RNAi-Ready pSIREN-RetroQ-ZsGreen Vector mediated recombinant pshRNA vectors in bovine granulosa cells (bGCs). The results showed that transfection with FST-1 and FST-2 vectors significantly down-regulated mRNA and protein expressions of follistatin by 51% (P = 0.0093) and 72% (P = 0.0078) respectively. After down-regulation of FST in bGCs, cell cycle was arrested at S-phase (9.2 ± 0.6 vs 12.5 ± 0.2, P = 0.0055), and apoptosis was significantly (21.3 ± 2.7 vs 13.9 ± 2.5, P = 0.0051) increased. These findings were further verified by down-regulation of protein level of B-cell leukemia/lymphoma 2 (Bcl2, P = 0.0423), and up-regulation of caspase-3 (P = 0.0362), p21 (P = 0.0067) and mRNA levels of Bcl2-associated X protein (Bax, P = 0.041). Knockdown of FST in bGCs significantly increased activin A concentration in culture medium, while level of estradiol (E2) was suppressed without affecting progesterone production. In addition, mRNA levels of all activin receptor subtypes [activin rece...Continue Reading

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Citations

Oct 24, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Larissa M CoutinhoFernando M Reis
Dec 15, 2015·The Journal of Reproduction and Development·Lei YangBaohua Ma
Aug 15, 2017·Molecular Reproduction and Development·Shu-Juan WangLi-Guo Yang
Nov 15, 2017·Reproduction in Domestic Animals = Zuchthygiene·Z GuoH Wu
Sep 12, 2019·Marine Genomics·Paola González-KotherDario Moraga
Jun 3, 2021·International Journal of Molecular Sciences·Malgorzata J McEvoyDariusz J Skarzynski

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