Disruption of four kinesin genes in dictyostelium.

BMC Cell Biology
Dilip K NagMichael P Koonce

Abstract

Kinesin and dynein are the two families of microtubule-based motors that drive much of the intracellular movements in eukaryotic cells. Using a gene knockout strategy, we address here the individual function(s) of four of the 13 kinesin proteins in Dictyostelium. The goal of our ongoing project is to establish a minimal motility proteome for this basal eukaryote, enabling us to contrast motor functions here with the often far more elaborate motor families in the metazoans. We performed individual disruptions of the kinesin genes, kif4, kif8, kif10, and kif11. None of the motors encoded by these genes are essential for development or viability of Dictyostelium. Removal of Kif4 (kinesin-7; CENP-E family) significantly impairs the rate of cell growth and, when combined with a previously characterized dynein inhibition, results in dramatic defects in mitotic spindle assembly. Kif8 (kinesin-4; chromokinesin family) and Kif10 (kinesin-8; Kip3 family) appear to cooperate with dynein to organize the interphase radial microtubule array. The results reported here extend the number of kinesin gene disruptions in Dictyostelium, to now total 10, among the 13 isoforms. None of these motors, individually, are required for short-term viability...Continue Reading

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Citations

Nov 20, 2012·Cellular and Molecular Life Sciences : CMLS·Irina TikhonenkoMichael P Koonce
Aug 25, 2015·Cellular and Molecular Life Sciences : CMLS·Irina TikhonenkoMichael P Koonce
Aug 21, 2008·Cell Motility and the Cytoskeleton·Irina TikhonenkoMichael P Koonce
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Jan 22, 2013·Biochemical and Biophysical Research Communications·Süleyman KösemAnnette Müller-Taubenberger
Apr 13, 2018·Cells·Michael P Koonce, Irina Tikhonenko
May 9, 2019·Molecular Biology of the Cell·Jacob OdellMichael Koonce
Apr 11, 2020·Biomolecules·Michael P Koonce, Irina Tikhonenko

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Methods Mentioned

BETA
environmental stresses
gene knockouts
PCR
light microscopy
gene knockout

Software Mentioned

Excel
ImageJ
Adobe Photoshop

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