Disruption of ku70 involved in non-homologous end-joining facilitates homologous recombination but increases temperature sensitivity in the phytopathogenic fungus Penicillium digitatum

Fungal Biology
Mónica GandíaJose F Marcos

Abstract

The dominant mechanism to repair double-stranded DNA breaks in filamentous fungi is the non-homologous end joining (NHEJ) pathway, and not the homologous recombination (HR) pathway that operates in the mutation of genes by replacement of target DNA for selection cassettes. The key to improve HR frequency is the inactivation of the NHEJ pathway by eliminating components of its Ku70/80 heterodimeric complex. We have obtained ku70 mutants of Penicillium digitatum, the main citrus postharvest pathogen. The increased efficiency of HR in Δku70 strains was demonstrated by the generation of mutants in two different chitin synthase genes (PdchsII and PdchsV). P. digitatum Δku70 strains showed no differences from the parental strain in vegetative growth, asexual development or virulence to citrus fruit, when experiments were conducted at the optimal temperature of 24°C. However, growth of Δku70 strains at temperatures higher than 24°C demonstrated a detrimental effect in axenic growth and conidia production. These observations are in agreement with previous studies describing differences between ku70 mutants and their parental strains in some fungal species, and must be taken into account for future applications of the Δku approach to in...Continue Reading

References

Nov 15, 2001·The EMBO Journal·H van AttikumP J Hooykaas
May 4, 2004·Nature Reviews. Molecular Cell Biology·Jessica A Downs, Stephen P Jackson
Aug 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuuko NinomiyaHirokazu Inoue
Nov 13, 2004·FEMS Microbiology Reviews·Zuzana DudásováMiroslav Chovanec
Feb 14, 2006·Molecular Genetics and Genomics : MGG·Tadashi TakahashiYasuji Koyama
Feb 6, 2007·Journal of Biotechnology·Vera MeyerArthur F J Ram
Oct 7, 2008·Journal of Zhejiang University. Science. B·Ji-Ye Wang, Hong-Ye Li
Jan 13, 2011·Current Microbiology·Jinxiang ZhangHuaming Wang
Jun 28, 2011·Fungal Genetics and Biology : FG & B·Takehito NakazawaTakashi Kamada
Aug 7, 2013·Applied Microbiology and Biotechnology·Tianyuan ZhangHongye Li
Feb 11, 2014·Journal of Zhejiang University. Science. B·Qian XuHong-ye Li
Aug 8, 2014·Molecular Plant Pathology·Mario López-PérezLuis González-Candelas
Dec 23, 2014·Research in Microbiology·Mingshuang WangHongye Li
Feb 6, 2015·Nucleic Acids Research·Carmen de Sena-TomásJosé Pérez-Martín
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·Victoria L Fell, Caroline Schild-Poulter

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Citations

Apr 25, 2019·Applied Microbiology and Biotechnology·Ying DingXiao-Jun Ji
Jul 31, 2019·Applied Microbiology and Biotechnology·Jesús Urbar-UlloaJorge Verdín
Aug 18, 2020·Journal of Microbiological Methods·Xin-Ai ChenYong-Quan Li

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