PMID: 9177159Jun 10, 1997Paper

Disruption of lysosomal targeting is associated with insecticidal potency of juvenile hormone esterase

Proceedings of the National Academy of Sciences of the United States of America
B C BonningB D Hammock

Abstract

Juvenile hormone esterase (JHE; EC 3.1.1.1), which is intrinsically involved in regulation of development of some insect larvae, is rapidly removed from the hemolymph by the pericardial cells. Lys-29 and Lys-524, which are implicated in the degradation of JHE, were mutated to Arg. Neither the half-life of the modified JHE in the hemolymph nor the catalytic parameters were changed significantly, but when combined, these mutations resulted in apparent failure of lysosomal targeting in the pericardial cell complex. A hypothesis for the mechanism of reduced efficiency of lysosomal targeting is presented. Infection of larvae with a recombinant baculovirus expressing the modified JHE resulted in a 50% reduction in feeding damage compared with larvae infected with the wild-type virus, thus demonstrating improved properties as a biological insecticide. These data demonstrate that alteration of specific residues of JHE that disrupted lysosomal targeting, dramatically increased the insecticidal activity of this protein.

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Citations

May 23, 2001·Archives of Biochemistry and Biophysics·A MiliarF G Hegardt
Jun 29, 2000·Biochemical and Biophysical Research Communications·K Kadono-OkudaG Jones
May 24, 2005·Cell and Tissue Research·Salvador Hernández-MartínezFernando G Noriega
Mar 30, 1978·Biochimica Et Biophysica Acta·D W Seccombe Novak
May 29, 2003·Journal of Insect Physiology·K Richter, M Gronert
Jun 14, 2000·Insect Biochemistry and Molecular Biology·B A ThomasB D Hammock
Dec 5, 2000·Insect Biochemistry and Molecular Biology·M ShanmugaveluB C Bonning
Aug 31, 2010·Journal of Pesticide Science·Shizuo G Kamita, Bruce D Hammock
Dec 14, 2011·Viruses·Jonathan E BreitenbachHolly J R Popham
Jun 7, 2011·General and Comparative Endocrinology·Naoki OkamotoAkira Mizoguchi
Aug 5, 2008·Journal of Insect Physiology·Chie OwaMasao Nagata
Nov 21, 2007·Journal of Insect Physiology·H Frederik Nijhout, Michael C Reed
Dec 1, 1992·Analytical Biochemistry·A SzékácsB D Hammock
Sep 26, 2006·Advances in Virus Research·A Bora InceogluBruce D Hammock
Nov 7, 2001·Pest Management Science·A B InceogluB D Hammock
Jun 16, 2007·Archives of Insect Biochemistry and Physiology·Manabu KamimuraMakoto Kiuchi

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