Disruption of PHO13 improves ethanol production via the xylose isomerase pathway

AMB Express
Takahiro BambaAkihiko Kondo

Abstract

Xylose is the second most abundant sugar in lignocellulosic materials and can be converted to ethanol by recombinant Saccharomyces cerevisiae yeast strains expressing heterologous genes involved in xylose assimilation pathways. Recent research demonstrated that disruption of the alkaline phosphatase gene, PHO13, enhances ethanol production from xylose by a strain expressing the xylose reductase (XR) and xylitol dehydrogenase (XDH) genes; however, the yield of ethanol is poor. In this study, PHO13 was disrupted in a recombinant strain harboring multiple copies of the xylose isomerase (XI) gene derived from Orpinomyces sp., coupled with overexpression of the endogenous xylulokinase (XK) gene and disruption of GRE3, which encodes aldose reductase. The resulting YΔGP/XK/XI strain consumed 2.08 g/L/h of xylose and produced 0.88 g/L/h of volumetric ethanol, for an 86.8 % theoretical ethanol yield, and only YΔGP/XK/XI demonstrated increase in cell concentration. Transcriptome analysis indicated that expression of genes involved in the pentose phosphate pathway (GND1, SOL3, TAL1, RKI1, and TKL1) and TCA cycle and respiratory chain (NDE1, ACO1, ACO2, SDH2, IDH1, IDH2, ATP7, ATP19, SDH4, SDH3, CMC2, and ATP15) was upregulated in the YΔGP...Continue Reading

References

Jan 1, 1992·Current Genetics·D C ChenT T Kuo
Feb 19, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Katleen De PreterJo Vandesompele
Dec 14, 2004·Bioresource Technology·Nathan MosierMichael Ladisch
Mar 23, 2005·FEMS Yeast Research·Lars M BlankUwe Sauer
Feb 6, 2007·Applied and Environmental Microbiology·Haiying NiThomas W Jeffries
May 26, 2007·Biotechnology Advances·Byron C H Chu, Hung Lee
Aug 19, 2007·Applied and Environmental Microbiology·Chenfeng Lu, Thomas Jeffries
Jun 24, 2009·Current Opinion in Biotechnology·J H Van Vleet, T W Jeffries
Jul 3, 2009·Applied Microbiology and Biotechnology·Akinori MatsushikaShigeki Sawayama
Jan 28, 2012·Journal of Bioscience and Bioengineering·Hiroko KatoAkihiko Kondo
Oct 15, 2014·Biotechnology and Bioengineering·Thomas M Wasylenko, Gregory Stephanopoulos

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Citations

Oct 13, 2016·Applied Microbiology and Biotechnology·Haibo LiHal S Alper
Apr 14, 2017·G3 : Genes - Genomes - Genetics·Karin Mittelman, Naama Barkai
Jun 30, 2019·Yeast·Margareth Andrea PatiñoBoris U Stambuk
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Buli SuHonghui Zhu
Nov 19, 2018·Applied Microbiology and Biotechnology·Jyumpei KobayashiAkihiko Kondo

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Datasets Mentioned

BETA
GSE73814

Methods Mentioned

BETA
PCR
chip

Software Mentioned

Metabolome
MassHunter Workstation Data Acquisition
MassHunter Quantitative Analysis

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