Disruption of Plasmodium berghei merozoite surface protein 7 gene modulates parasite growth in vivo.

Blood
Rita TewariA A Holder

Abstract

Merozoite invasion of red blood cells is crucial to the development of the parasite that causes malaria. Merozoite surface proteins (MSPs) mediate the first interaction between parasite and erythrocyte. In Plasmodium falciparum, they include a complex of products from at least 3 genes (msp1, msp6, and msp7), one of which, msp7, is part of a gene family containing 3 and 6 adjacent members in Plasmodium yoelii and Plasmodium falciparum, respectively. We have identified and disrupted msp7 in the Plasmodium berghei gene family. The protein is expressed in schizonts and colocalizes with MSP1. The synthesis and processing of MSP1 was unaffected in the parasite with the disrupted gene (MSP7ko). Disruption of msp7 was not lethal but affected blood-stage parasite growth. MSP7ko parasites initially grew more slowly than wild-type parasites. However, when reticulocytes were prevalent, the rate of increase in parasitemia was similar, suggesting that MSP7ko parasites prefer to invade and grow within reticulocytes.

References

Nov 1, 1994·Experimental Parasitology·S A Ogun, A A Holder
Jul 1, 1994·Molecular and Biochemical Parasitology·W H StaffordA A Holder
Oct 1, 1996·Molecular and Biochemical Parasitology·W H StaffordM J Blackman
Feb 12, 1998·Methods : a Companion to Methods in Enzymology·A P WatersC J Janse
Feb 13, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alan F CowmanBrendan S Crabb
Oct 5, 2002·Science·Alan F Cowman, Brendan S Crabb

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Citations

Dec 17, 2009·Hematology·Kasturi Haldar, Narla Mohandas
Dec 14, 2012·Future Medicinal Chemistry·Isabelle S LucetChristian Doerig
May 11, 2010·International Journal for Parasitology·Madhusudan Kadekoppala, Anthony A Holder
Nov 4, 2006·Biochemical and Biophysical Research Communications·Alvaro MonguiManuel A Patarroyo
Jul 17, 2007·Molecular Microbiology·Jayasree IyerPeter R Preiser
Aug 26, 2010·Molecular Microbiology·Matthew A ChildMichael J Blackman
Jun 6, 2015·Malaria Journal·Abigail J PerrinGavin J Wright
Dec 25, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Diego Garzón-OspinaManuel A Patarroyo
Dec 19, 2014·Frontiers in Microbiology·Priyanka FernandesAnn-Kristin Mueller
Aug 31, 2006·The Journal of Biological Chemistry·Christian W KauthHermann Bujard
Nov 30, 2016·BMC Evolutionary Biology·Diego Garzón-OspinaManuel A Patarroyo

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