Disruption of Rho signal transduction upon cell detachment

Journal of Cell Science
Xiang-Dong RenRichard A F Clark

Abstract

Serum-soluble factors play a dominant role in the activation of the small GTPase RhoA. Cell adhesion also modulates RhoA activity but the effect is modest in the absence of serum. Here, we show that cell adhesion is required for serum-stimulated Rho signal transduction leading to myosin light chain (MLC) phosphorylation. Characterization of Rho-kinase substrates revealed that diphosphorylation of MLC at Thr-18 and Ser-19 (ppMLC(T18/S19)) and phosphorylation of the myosin-binding subunit (MBS) of myosin phosphatase at Thr-853 (pMBS(T853)) were mostly Rho and Rho-kinase dependent in attached fibroblasts. MLC monophosphorylation at Ser-19 (pMLC(S19)) was partially dependent on Rho kinase, whereas phosphorylation of MBS at Thr-696 (pMBS(T696)) and phosphorylation of CPI-17 at Thr-38 (pCPI-17(T38)) were mostly Rho-kinase independent. Cell detachment caused a significant reduction in pMLC(S19) and a more dramatic decrease of ppMLC(T18/S19) without inhibiting RhoA. pMBS(T853), pMBS(T696) and pCPI-17(T38) were not significantly reduced, suggesting that myosin-phosphatase activity was little changed. Cells expressing active RhoA (RhoA(V14)) or Rho-kinase catalytic domain maintained elevated pMBS(T853) upon detachment but failed to suppo...Continue Reading

References

Jan 1, 1992·Current Topics in Microbiology and Immunology·K AktoriesG Koch
Feb 1, 1991·Current Opinion in Cell Biology·J R Sellers
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·M S Kolodney, E L Elson
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M A SchwartzM H Ginsberg
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Sep 6, 1996·The Journal of Biological Chemistry·M W RenshawM A Schwartz
Apr 21, 1997·The Journal of Cell Biology·R L KlemkeD A Cheresh
Feb 7, 1998·Science·A Hall
Jan 15, 1999·Acta Physiologica Scandinavica·D J Hartshorne
Jan 26, 1999·Annals of the New York Academy of Sciences·S MiyamotoK M Yamada
Dec 22, 1999·The Journal of Biological Chemistry·J FengT Nakano
Dec 28, 1999·Science·A R Horwitz, J T Parsons
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Aug 10, 2000·The Journal of Cell Biology·N K NorenB Kreft
Oct 19, 2000·Methods in Enzymology·X D Ren, M A Schwartz
Oct 19, 2000·Methods in Enzymology·S NarumiyaM Uehata
Nov 30, 2000·The Journal of Biological Chemistry·K E Kamm, J T Stull
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·J A MacDonaldT A Haystead
Nov 24, 2001·The Journal of Biological Chemistry·Constantinos G BroustasUsha Kasid
Jan 16, 2002·Current Opinion in Genetics & Development·Alan K HoweR L Juliano
Apr 11, 2002·Nature Cell Biology·Martin A Schwartz, Mark H Ginsberg
Apr 11, 2002·Nature Cell Biology·Cindy K Miranti, Joan S Brugge
Oct 3, 2002·Biochemical and Biophysical Research Communications·Norio TakizawaMitsuo Ikebe
Jan 22, 2003·The Biochemical Journal·Jenny BainPhilip Cohen
May 30, 2003·Molecular and Cellular Biology·Kirsi RientoAnne J Ridley
Jun 13, 2003·Molecular Biology of the Cell·Shigeko YamashiroFumio Matsumura

❮ Previous
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Citations

Dec 20, 2008·The Journal of Biological Chemistry·Ronald L NepplAvril V Somlyo
Sep 10, 2010·Journal of the Royal Society, Interface·Kazuo KatohYasuko Noda
Apr 26, 2011·Computer Methods in Biomechanics and Biomedical Engineering·Sangyoon J Han, Nathan J Sniadecki
Jan 16, 2014·Journal of Clinical Biochemistry and Nutrition·Masao YamasakiKazuo Nishiyama
Feb 4, 2012·Experimental Eye Research·Ariadna Yolanda Ruiz-LoredoAna María López-Colomé
Sep 7, 2012·The Journal of Investigative Dermatology·Jelena GrahovacAlan Wells
Aug 4, 2007·Experimental Cell Research·Kiran BhadrirajuChristopher S Chen
Jul 17, 2007·Biochemical and Biophysical Research Communications·Teerin T MeckmongkolLivingston Van De Water
Aug 18, 2006·Experimental Eye Research·Yang LiuTeruo Nishida
Apr 6, 2006·Experimental Cell Research·Kirsty D SmithDouglas A Lauffenburger
Apr 24, 2012·Cellular Signalling·Hiroto Okayama
Oct 2, 2007·Seminars in Cell & Developmental Biology·Asier EcharriMiguel A Del Pozo
Nov 13, 2012·Biomaterials·Yi-Hsin ChenTai-Horng Young
Jun 21, 2005·The Journal of Biological Chemistry·Ruixue WangXiang-Dong Ren
Aug 9, 2007·The Journal of Biological Chemistry·Eunok Im, Andrius Kazlauskas
Dec 15, 2007·The Journal of Biological Chemistry·Daisuke UmedaHirofumi Tachibana
Dec 2, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Navneeta RathorJaladanki N Rao
Sep 5, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hee Kyoung ChungJaladanki N Rao
Aug 8, 2019·Angewandte Chemie·Yizhe ZhangAmy E Herr
Sep 22, 2006·American Journal of Physiology. Cell Physiology·Yong-Bae KimJung Weon Lee
Dec 24, 2010·Virulence·Marcus B JonesMartin J Blaser
Jul 27, 2005·Biochemical Society Transactions·A Grande-GarcíaM A Del Pozo
Dec 22, 2004·The Journal of Biological Chemistry·Hua Zhou, Randall H Kramer
Nov 10, 2017·Lab on a Chip·Elaine J Su, Amy E Herr
Oct 12, 2007·Journal of Cell Science·Stéphanie Pellegrin, Harry Mellor

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