Disruption of Rhodopsin Dimerization with Synthetic Peptides Targeting an Interaction Interface.

The Journal of Biological Chemistry
Beata JastrzebskaKrzysztof Palczewski

Abstract

Although homo- and heterodimerizations of G protein-coupled receptors (GPCRs) are well documented, GPCR monomers are able to assemble in different ways, thus causing variations in the interactive interface between receptor monomers among different GPCRs. Moreover, the functional consequences of this phenomenon, which remain to be clarified, could be specific for different GPCRs. Synthetic peptides derived from transmembrane (TM) domains can interact with a full-length GPCR, blocking dimer formation and affecting its function. Here we used peptides corresponding to TM helices of bovine rhodopsin (Rho) to investigate the Rho dimer interface and functional consequences of its disruption. Incubation of Rho with TM1, TM2, TM4, and TM5 peptides in rod outer segment (ROS) membranes shifted the resulting detergent-solubilized protein migration through a gel filtration column toward smaller molecular masses with a reduced propensity for dimer formation in a cross-linking reaction. Binding of these TM peptides to Rho was characterized by both mass spectrometry and a label-free assay from which dissociation constants were calculated. A BRET (bioluminescence resonance energy transfer) assay revealed that the physical interaction between Rh...Continue Reading

References

Jan 1, 1982·Methods in Enzymology·D S Papermaster
Apr 22, 1993·Nature·G F SchertlerR Henderson
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·G F Schertler, P A Hargrave
Apr 4, 1997·Science·K R MacKenzieD M Engelman
Apr 12, 2000·Biochemistry·W C Wimley, S H White
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Aug 5, 2000·Science·K PalczewskiM Miyano
Apr 3, 2001·Nature Reviews. Neuroscience·M Bouvier
Dec 24, 2002·The Journal of Biological Chemistry·Wen GuoJonathan A Javitch
Jan 10, 2003·Nature·Dimitrios FotiadisKrzysztof Palczewski
Mar 29, 2003·The Journal of Biological Chemistry·Yan LiangAndreas Engel
Nov 20, 1953·The Journal of General Physiology·G WALD, P K BROWN
Nov 7, 2003·Nature·Marc ChabreHelen Saibil
Nov 11, 2003·Life Sciences·Graeme MilliganJuan J Carrillo
Nov 13, 2003·Vision Research·Eva RamonPere Garriga
Jan 13, 2004·Nature Immunology·Patricia Hernanz-FalcónMario Mellado
Apr 28, 2004·FEBS Letters·Dimitrios FotiadisKrzysztof Palczewski
Jun 2, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Slawomir FilipekKrzysztof Palczewski
Aug 27, 2004·The EMBO Journal·Jonathan J RuprechtGebhard F X Schertler
Oct 19, 2004·The Journal of Biological Chemistry·Beata JastrzebskaKrzysztof Palczewski
Dec 14, 2004·Proteins·Wataru Nemoto, Hiroyuki Toh
Jul 6, 2005·Biochemistry·Marc Chabre, Marc le Maire
Jan 13, 2006·Nature Chemical Biology·Anatoli IanoulJohn Paul Pezacki
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Steven E MansoorDavid L Farrens
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Parvathi KotaH Gobind Khorana
Feb 24, 2006·The Journal of Biological Chemistry·Beata JastrzebskaKrzysztof Palczewski
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·David SalomKrzysztof Palczewski
Apr 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Matthew R WhortonRoger K Sunahara
May 31, 2007·The Journal of Cell Biology·Stephan NickellKrzysztof Palczewski
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jim F WhiteReinhard Grisshammer
Nov 24, 2007·The Journal of Biological Chemistry·Matthew R WhortonRoger K Sunahara
Mar 7, 2008·Pharmacological Reviews·Daniel J MüllerKrzysztof Palczewski
Mar 18, 2008·EMBO Reports·Filippo ManciaWayne A Hendrickson
Mar 7, 2009·Biochemical and Biophysical Research Communications·Dusan VobornikJohn Paul Pezacki
Mar 10, 2009·Trends in Pharmacological Sciences·Marc ChabreBruno Antonny
Mar 25, 2009·British Journal of Pharmacology·Graeme Milligan
Oct 20, 2009·Biochemical and Biophysical Research Communications·Won-Kyu LeeYeon Gyu Yu

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Citations

Dec 5, 2015·Frontiers in Molecular Neuroscience·Karl-Wilhelm Koch, Daniele Dell'Orco
Jul 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Tao ZhangYingbin Fu
Aug 6, 2016·Journal of the American Chemical Society·Rabindra V ShivnaraineClaudiu C Gradinaru
Nov 9, 2016·Frontiers in Physiology·Stefan Gahbauer, Rainer A Böckmann
Nov 14, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yuanyuan ChenKrzysztof Palczewski
Mar 30, 2018·Nature Communications·Gemma NavarroSergi Ferré
Dec 3, 2017·Scientific Reports·Kalpana PandeyAnant K Menon
Oct 4, 2016·Nature Communications·Birgit PloierAnant K Menon
Feb 1, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah O K MakBilly K C Chow
May 19, 2018·Nature Communications·Yuanyuan ChenKrzysztof Palczewski
May 24, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tamar GetterKrzysztof Palczewski
Oct 9, 2019·International Journal of Molecular Sciences·Maria GalloDavid Andreu
Dec 28, 2019·Journal of Computational Chemistry·Lucas WäschenbachHolger Gohlke
Apr 16, 2016·The Journal of Biological Chemistry·John D PedianiGraeme Milligan
Apr 14, 2016·Biochemical Society Transactions·Roberto MaggioMarco Scarselli
Aug 11, 2019·The Journal of Biological Chemistry·Dorothy Yanling ZhaoKrzysztof Palczewski
Dec 15, 2019·International Journal of Molecular Sciences·Joseph T Ortega, Beata Jastrzebska
Sep 15, 2018·Frontiers in Pharmacology·Dasiel O Borroto-EscuelaJens Carlsson
Feb 7, 2021·Drug Discovery Today·Léopold ThabaultRaphaël Frédérick
Feb 14, 2021·Cellular and Molecular Life Sciences : CMLS·Rafael FrancoLeonardo Pardo
Feb 17, 2021·Pflügers Archiv : European journal of physiology·Paul S-H Park
Feb 17, 2021·Journal of Cell Science·Xiaoming FangJarema J Malicki
Mar 27, 2021·Journal of Biological Rhythms·Brian A UptonRichard A Lang
Apr 24, 2021·Journal of Medicinal Chemistry·Maria GalloDavid Andreu
May 20, 2021·Scientific Reports·George KhelashviliAnant K Menon
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pol Fernandez-GonzalezPere Garriga
Jan 4, 2017·Biochemistry·Beata JastrzebskaAdam W Smith
Oct 8, 2020·Biochemistry·Joshua A JesinCharles M Deber
Jan 20, 2019·Neuropharmacology·Rory Sleno, Terence E Hébert

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