Disruption of the Crithidia fasciculata KAP1 gene results in structural rearrangement of the kinetoplast disc

Molecular and Biochemical Parasitology
J LukesD S Ray

Abstract

The mitochondrial DNA (kinetoplast DNA) in trypanosomatids exists as a highly organized nucleoprotein structure with the DNA consisting of thousands of interlocked circles. Four H1 histone-like proteins (KAP1, 2, 3 and 4) are associated with the kinetoplast DNA in the trypanosomatid Crithidia fasciculata. We have disrupted both alleles of the KAP1 gene in this diploid protozoan and shown that expression of the KAP1 protein is eliminated. The mutant strain is viable but has substantial rearrangement of the kinetoplast structure. Expression of the KAP1 protein from an episome restored expression of the KAP1 protein in the mutant strain and also restored a normal kinetoplast structure. These studies provide evidence that the KAP1 protein is involved in kinetoplast DNA organization in vivo but is nonessential for cell viability.

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Citations

Oct 11, 2011·The Journal of Biological Chemistry·Irit KapellerJoseph Shlomai
Nov 29, 2002·Eukaryotic Cell·Julius LukesPaul T Englund
Dec 29, 2010·PLoS Pathogens·Arnab Roy ChowdhuryPaul T Englund
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Krishna Murari SinhaDan S Ray
Nov 2, 2004·Parasitology Research·Danielle P CavalcantiMaria Cristina M Motta
Feb 6, 2010·Molecular Microbiology·David F BruhnMichele M Klingbeil
Apr 8, 2018·Journal of Cell Science·Simona AmodeoTorsten Ochsenreiter
Oct 3, 2018·Future Science OA·Aline A Zuma, Wanderley de Souza
Jan 17, 2021·Scientific Reports·Nurit YaffeJoseph Shlomai

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