Disruption of the Eng18B ENGase gene in the fungal biocontrol agent Trichoderma atroviride affects growth, conidiation and antagonistic ability.

PloS One
Mukesh K DubeyMagnus Karlsson

Abstract

The recently identified phylogenetic subgroup B5 of fungal glycoside hydrolase family 18 genes encodes enzymes with mannosyl glycoprotein endo-N-acetyl-β-D-glucosaminidase (ENGase)-type activity. Intracellular ENGase activity is associated with the endoplasmic reticulum associated protein degradation pathway (ERAD) of misfolded glycoproteins, although the biological relevance in filamentous fungi is not known. Trichoderma atroviride is a mycoparasitic fungus that is used for biological control of plant pathogenic fungi. The present work is a functional study of the T. atroviride B5-group gene Eng18B, with emphasis on its role in fungal growth and antagonism. A homology model of T. atroviride Eng18B structure predicts a typical glycoside hydrolase family 18 (αβ)(8) barrel architecture. Gene expression analysis shows that Eng18B is induced in dual cultures with the fungal plant pathogens Botrytis cinerea and Rhizoctonia solani, although a basal expression is observed in all growth conditions tested. Eng18B disruption strains had significantly reduced growth rates but higher conidiation rates compared to the wild-type strain. However, growth rates on abiotic stress media were significantly higher in Eng18B disruption strains compa...Continue Reading

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Citations

Nov 2, 2015·Molecular Genetics and Genomics : MGG·Mukesh DubeyMagnus Karlsson
Jan 5, 2014·Fungal Genetics and Biology : FG & B·Georgios D TzelepisMagnus Karlsson
Feb 13, 2016·Microbiology and Molecular Biology Reviews : MMBR·Monika SchmollAlfredo Herrera-Estrella
Mar 11, 2017·Microbiology Spectrum·Magnus KarlssonSusanne Zeilinger
Sep 23, 2021·Microbiology Spectrum·Edoardo PiomboMukesh Dubey

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Datasets Mentioned

BETA
JF300121-JF300127

Methods Mentioned

BETA
PCR
glycosylation
environmental stress

Software Mentioned

Clustal W
SMART
Leica application suite
SignalP
InterProScan
Conserved Domain Search
Signal P
TMHMM
PI Fungal Predictor
Statistica

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