Abstract
Excitotoxicity and neuronal death following epilepsy involve α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs). It forms a protein complex with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and co-internalizes upon activation of AMPA receptors after epilepsy. Disruption of the GluA2/GAPDH complex with an interfering peptide, TAT-GluA2NT1-3-2, protects cells against AMPAR-mediated excitotoxicity, which have been identified in in-vitro and in-vivo models of brain ischemia. We postulated that disruption of the GluA2/GAPDH interaction with the TAT-GluA2NT1-3-2 peptide would also protect against AMPAR-induced neuronal injury in an in-vivo model of status epilepticus (SE). In the present study, we divided pilocarpine-induced SE Wistar rats into three main groups: the TAT-GluA2NT1-3-2 peptide group, the TAT-GluA2NT-scram peptide group, and the normal saline group, and injected different doses of peptides stereotaxically into the hippocampus of SE rats to investigate whether the GluA2/GAPDH interaction could be disrupted by our TAT-GluA2NT1-3-2 peptide and determine its most appropriate dose. Then, the dose was administered stereotaxically at different time points after SE to determine the best administration t...Continue Reading
References
Dec 16, 1997·Brain : a Journal of Neurology·G W MathernW J Peacock
Sep 8, 1999·Science·S R SchwarzeS F Dowdy
Jul 4, 2001·Neuroreport·Y FukuharaK Nakashima
Mar 19, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·C Anthony AltarMichael G Palfreyman
Jul 6, 2006·The European Journal of Neuroscience·Brenda E PorterAmy R Brooks-Kayal
Nov 27, 2007·Epilepsy Research·Roland WeicznerAndrás Mihály
Aug 8, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sanjay N RakhadeFrances E Jensen
Apr 1, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·H Steve WhiteGrzegorz Bulaj
Mar 17, 2010·Pflügers Archiv : European journal of physiology·Anthony Lau, Michael Tymianski
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine
Feb 18, 2012·Current Medicinal Chemistry·P Marmiroli, G Cavaletti
Mar 1, 2012·Journal of Molecular Neuroscience : MN·Juan ChengChen Zhang
Apr 25, 2012·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Yue HuXiaoPing Zhang
Apr 28, 2012·Molecular Brain·Min WangFang Liu
Jun 28, 2012·Neuropharmacology·Jonathan E HarmsCraig Jamieson
Jul 14, 2012·Expert Opinion on Investigational Drugs·Emilio RussoGiovambattista De Sarro
Jan 31, 2013·Neurobiology of Disease·Dongxu ZhaiFang Liu
Jan 7, 2014·Expert Opinion on Therapeutic Targets·Rita CitraroEmilio Russo
Sep 11, 2017·Epilepsy Research·Jan-Øyvind LorgenSvend Davanger