Disruption of the maxi-K-caveolin-1 interaction alters current expression in human myometrial cells.

Reproductive Biology and Endocrinology : RB&E
Adam M BrainardSarah K England

Abstract

One determinant of the total K+ myometrial smooth muscle cell (MSMC) current is the large conductance, calcium- and voltage-activated potassium channel (maxi-K channel). This channel provides a repolarizing current in response to excitatory stimuli, most notably in response to increases in the levels of intracellular Ca2+, and blocking the channel by pharmacological means induces the depolarization of MSMCs and also enhances contraction strength. In MSMCs, maxi-K channels can reside in the caveolae, where they associate with the scaffolding protein caveolin-1 (cav-1). The aim of this study was to investigate the consequences of this interaction - more specifically, how disruption of the association between the maxi-K channel and cav-1 may influence the current expression and excitability of myometrial cells - with the aim of better understanding the mechanisms that underlie the regulation of normal and aberrant uterine function. Myometrial biopsies were collected from women undergoing elective C-sections. From these samples, myometrial cells were isolated, cultured, infected with a virus containing either caveolin-1 (cav-1) siRNA or scrambled cav-1 siRNA, and finally subjected to patch-clamp analysis. Mutant caveolin-binding si...Continue Reading

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Citations

Jun 22, 2010·Histochemistry and Cell Biology·K BarthM Kasper
Jun 4, 2010·The Journal of Physiology·Caroline Dart
Jun 5, 2012·Journal of Molecular and Cellular Cardiology·Andrea BarbutiDario Difrancesco
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Aug 19, 2014·Frontiers in Physiology·Ramón A LorcaSarah K England
Nov 8, 2020·Annual Review of Physiology·Susan Wray, Sarah Arrowsmith

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Methods Mentioned

BETA
Cesarean section
immunoprecipitation
confocal microscopy
Transfections
density gradient centrifugation

Software Mentioned

SPSS
SigmaPlot
pClamp

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