Disruption of the membrane-bound alcohol dehydrogenase-encoding gene improved glycerol use and dihydroxyacetone productivity in Gluconobacter oxydans

Bioscience, Biotechnology, and Biochemistry
Hiroshi HabeKeiji Sakaki

Abstract

Dihydroxyacetone (DHA) production from glycerol by Gluconobacter oxydans is an industrial form of fermentation, but some problems exist related to microbial DHA production. For example, glycerol inhibits DHA production and affects its biological activity. G. oxydans produces both DHA and glyceric acid (GA) from glycerol simultaneously, and membrane-bound glycerol dehydrogenase and membrane-bound alcohol dehydrogenases are involved in the two reactions, respectively. We discovered that the G. oxydans mutant DeltaadhA, in which the membrane-bound alcohol dehydrogenase-encoding gene (adhA) was disrupted, significantly improved its ability to grow in a higher concentration of glycerol and to produce DHA compared to a wild-type strain. DeltaadhA grew on 220 g/l of initial glycerol and produced 125 g/l of DHA during a 3-d incubation, whereas the wild-type did not. Resting DeltaadhA cells converted 230 g/l of glycerol aqueous solution to 139.7 g/l of DHA during a 3-d incubation. The inhibitory effect of glycerate sodium salt on DeltaadhA was investigated. An increase in the glycerate concentration at the beginning of growth resulted in decreases in both growth and DHA production.

References

Jan 1, 1994·Advances in Microbial Physiology·K MatsushitaO Adachi
Feb 8, 2002·Applied Microbiology and Biotechnology·K MatsushitaO Adachi
Nov 5, 2003·Bioprocess and Biosystems Engineering·D HekmatJ Fricke
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May 15, 2007·Applied Microbiology and Biotechnology·Cornelia GätgensUte Herrmann
Apr 5, 2008·Biotechnology Advances·Ruchi MishraAshok Kumar
Sep 9, 2008·Biotechnology Advances·Gervásio Paulo da SilvaJonas Contiero
Jul 22, 2009·Applied Microbiology and Biotechnology·Hiroshi HabeKeiji Sakaki
Aug 8, 2009·Bioscience, Biotechnology, and Biochemistry·Hiroshi HabeKeiji Sakaki
Oct 20, 2009·Applied and Environmental Microbiology·Hiroshi HabeKeiji Sakaki

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Citations

May 25, 2011·Journal of Oleo Science·Hiroshi HabeKeiji Sakaki
Oct 27, 2011·Journal of Oleo Science·Hiroshi HabeKeiji Sakaki
May 22, 2013·Biotechnology Letters·Anu Jose MattamSyed Shams Yazdani
Mar 14, 2018·ChemistryOpen·Rosaria CiriminnaMario Pagliaro

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