Disruption of the novel plant protein NEF1 affects lipid accumulation in the plastids of the tapetum and exine formation of pollen, resulting in male sterility in Arabidopsis thaliana

The Plant Journal : for Cell and Molecular Biology
Tohru AriizumiKinya Toriyama

Abstract

A novel male-sterile mutant of Arabidopsis thaliana was isolated by means of T-DNA tagging. Pollen abortion of the mutant was evident after microspore release, and pollen grains were completely absent at anthesis. Transmission electron microscope analysis revealed that primexine was coarsely developed, and that although sporopollenin was produced, it was not deposited onto the microspore plasma membrane. The sporopollenin that failed to be deposited aggregated and accumulated within the locule and on the locule wall. Finally, as no exine formation was observed, the mutant was named nef1. The plastoglobuli within the plastids of the tapetum were reduced, and lipid accumulation was considerably decreased. The mutant had a significantly altered leaf chloroplast ultrastructure and showed various growth defects. Lipid analysis revealed that the total lipid content in nef1 was lower than that in the wild type, which indicated that Nef1 was involved in lipid metabolism. Cloning of the full-length Nef1 indicated that the gene encodes a novel plant protein of 1123 amino acids with limited sequence similarities to membrane proteins or transporter-like proteins, and the NEF1 is predicted to be a plastid integral membrane protein. Motif an...Continue Reading

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Citations

Dec 25, 2009·Sexual Plant Reproduction·Anna A DobritsaRobert J Swanson
May 20, 2011·Plant Molecular Biology·Rachel A Okrent, Mary C Wildermuth
Jan 1, 2011·AoB Plants·Simon WallaceDavid J Beerling
Mar 27, 2009·Journal of Experimental Botany·Zoe A Wilson, Da-Bing Zhang
Sep 10, 2008·Plant & Cell Physiology·Toshiya SuzukiSumie Ishiguro
Oct 8, 2009·Plant & Cell Physiology·Makoto EndoMakiko Kawagishi-Kobayashi
May 9, 2014·Nature Communications·Yue LouZhong-Nan Yang
Feb 1, 2011·Annual Review of Plant Biology·Tohru Ariizumi, Kinya Toriyama
Mar 29, 2014·Protoplasma·Nina I Gabarayeva, Valentina V Grigorjeva
Sep 21, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Dabing ZhangLu Zhu

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