Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite development in the vector mosquito

Molecular and Biochemical Parasitology
Kazuhiko YanoShin-ichiro Kawazu

Abstract

To investigate the physiologic role of cytosolic 2-Cys peroxiredoxin of Plasmodium berghei (PbTPx-1), we infected the vector mosquito Anopheles stephensi with a parasite carrying a targeted knockout of pbtpx-1 (Prx-KO). The number of Prx-KO midgut oocysts at 14-15 days post-feeding (pf) was comparable to that of the parent strain (WT); however, the numbers of sporozoites that formed in midgut oocysts and accumulated in the salivary gland of Prx-KO-infected mosquitoes by 21 days pf were decreased to 10-20% and 3-10%, respectively, of those values in WT-infected mosquitoes. A higher frequency of DNA strand breaks was detected in Prx-KO oocysts than in WT oocysts. Sporozoites carrying the targeted disruption had reduced infectivity in mice; however, the knockout did not affect the ability of the sporozoite to reach the liver parenchyma and initiate exo-erythrocytic form (EEF) development. TPx-1 may be involved in development during exponentially multiplying stages, such as sporozoites and EEF.

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Sep 25, 2007·Parasitology International·Shin-ichiro KawazuShigeyuki Kano

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Citations

Nov 22, 2011·Antioxidants & Redox Signaling·Michael C GretesP Andrew Karplus
Jan 19, 2012·Antimicrobial Agents and Chemotherapy·Akiko KimuraShin-Ichiro Kawazu
Jan 15, 2014·PLoS Neglected Tropical Diseases·Daniela MastronicolaAlessandro Giuffrè
Sep 4, 2012·International Journal of Medical Microbiology : IJMM·Esther Jortzik, Katja Becker
Sep 9, 2010·Molecular Systems Biology·Germán PlataDennis Vitkup
Sep 4, 2014·Memórias do Instituto Oswaldo Cruz·Ryan C SmithMarcelo Jacobs-Lorena
Mar 6, 2015·Biological Chemistry·Mahsa RahbariEsther Jortzik
Sep 21, 2019·Scientific Reports·Christina BrandstaedterKatja Becker
Jan 19, 2021·Parasitology Research·Savitri TiwariNeelima Mishra
Nov 19, 2014·Biochemistry·Arden PerkinsP Andrew Karplus

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