Disruption of the thrombospondin-2 gene alters the lamellar morphology but does not permit vascularization of the adult mouse lumbar disc.

Arthritis Research & Therapy
Helen E GruberEdward N Hanley

Abstract

The biological basis for the avascular state of the intervertebral disc is not well understood. Previous work has suggested that the presence of thrombospondin-1 (TSP-1), a matricellular protein, in the outer annulus reflects a role for this protein in conferring an avascular status to the disc. In the present study we have examined thrombospondin-2 (TSP-2), a matricellular protein with recognized anti-angiogenic activity in vivo and in vitro. We examined both the location and expression of TSP-2 in the human disc, and its location in the disc and bordering soft tissues of 5-month-old normal wild-type (WT) mice and of mice with a targeted disruption of the TSP-2 gene. Immunohistochemistry and quantitative histology were utilized in this study. TSP-2 was found to be present in some, but not all, annulus cells of the human annulus and the mouse annulus. Although there was no difference in the number of disc cells in the annulus of TSP-2-null mice compared with that of WT animals, polarized light microscopy revealed a more irregular lamellar collagen structure in null mouse discs compared with WT mouse discs. Additionally, vascular beds at the margins of discs of TSP-2-null mice were substantially more irregular than those of WT a...Continue Reading

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Citations

Aug 31, 2011·Cold Spring Harbor Perspectives in Biology·Josephine C Adams, Jack Lawler
Jun 7, 2014·International Journal of Medical Sciences·Helen E GruberEdward N Hanley
May 31, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Jake BedoreCheryle A Séguin
Feb 13, 2015·Journal of the Royal Society, Interface·Maria MolinosMário A Barbosa
Jun 15, 2018·International Journal of Molecular Sciences·Ann-Kathrin SchubertMichael Sittinger

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Methods Mentioned

BETA
laser capture microdissection
light
light microscopy

Software Mentioned

GCOS Affymetrix GeneChip Operating System
SAS
GraphPad InStat

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