PMID: 2105746Jan 29, 1990Paper

Disruption of thymidylate synthesis and glycine-serine interconversion by L-methionine and L-homocystine in Raji cells

Biochimica Et Biophysica Acta
D Fell, J Selhub

Abstract

Excessive concentrations of L-methionine inhibited the folate-dependent de novo synthesis of thymidylic acid (TMP) in Raji cells, demonstrating the usefulness of this cell line for the study of methionine-folate antagonism. The effect was also produced by L-homocystine but not by other amino acids including D-methionine and L-ethionine, suggesting that this effect is exerted by a common intermediate of methionine and homocystine metabolism. L-Methionine, L-homocysteine, S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH) are not inhibitors of thymidylate synthase activity. On the other hand the capacity of the cells to incorporate serine 3-carbon and glycine 2-carbon into DNA is impaired by the presence of L-methionine or L-homocystine. Studies with cell-free extracts demonstrated that the glycine cleavage enzyme is inhibited by 45% by L-methionine, L-homocysteine, SAM or SAH. Serine hydroxymethylase on the other hand was slightly stimulated by these sulfur-containing compounds and this stimulation was shown to occur in the intact cell as well. These findings suggest that when levels of L-methionine metabolites are elevated, there is an increase in the use of glycine to maintain the intracellular concentration of serin...Continue Reading

References

Sep 2, 1975·Biochemical and Biophysical Research Communications·J D FinkelsteinA M Pick
Dec 1, 1974·Archives of Biochemistry and Biophysics·J D FinkelsteinB J Harris
Feb 1, 1969·The Journal of Clinical Investigation·S WaxmanV Herbert
Nov 1, 1966·Biochemistry·D Roberts
Nov 1, 1948·Proceedings of the Society for Experimental Biology and Medicine·J H BROWN, J B ALLISON

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Citations

May 1, 1996·Atherosclerosis·G BlundellN Tudball
Feb 27, 2004·Biochimica Et Biophysica Acta·Yuh-Shuh WangChung-Jui Tsai
Jan 19, 2006·European Journal of Clinical Nutrition·L Torres-SánchezL López-Carrillo
Aug 10, 2004·Nutrition Reviews·Patrick J Stover
Nov 26, 2020·International Journal of Molecular Sciences·Yee-Ling TanEn-Pei Isabel Chiang
Aug 2, 2005·Alcohol·Joel B Mason, Sang-Woon Choi

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