PMID: 8444170Mar 1, 1993Paper

Disruption of TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity

European Journal of Biochemistry
C De VirgilioA Wiemken

Abstract

Preparations of the trehalose-6-phosphate synthase/phosphatase complex from Saccharomyces cerevisiae contain three polypeptides with molecular masses 56, 100 and 130 kDa, respectively. Recently, we have cloned the gene for the 56-kDa subunit of this complex (TPS1) and found it to be identical with CIF1, a gene essential for growth on glucose and for the activity of trehalose-6-phosphate synthase. Peptide sequencing of the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex (TPS2) revealed one sequence to be 100% identical with the deduced amino acid sequence of the upstream region of PPH3 on the right arm of chromosome IV. This sequence was used to clone an upstream region of PPH3 containing an open reading frame of 2685 nucleotides, predicted to encode a polypeptide of 102.8 kDa. The N-terminal sequence, as well as three internal amino acid sequences, obtained from peptide sequencing of the 100-kDa subunit, were identical with specific regions of the deduced amino acid sequence. Thus, the sequence cloned represents TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex. Interestingly, a stretch of about 500 amino acids from the first part of TPS2 was 33% iden...Continue Reading

References

Jul 7, 1977·Molecular & General Genetics : MGG·K W van de Poll, D H Schamhart
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1978·Analytical Biochemistry·G F MiozzariR Hütter
Oct 21, 1991·FEBS Letters·K WinklerH Holzer
Oct 1, 1990·Antonie van Leeuwenhoek·A Wiemken
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Munro, T Maniatis
May 1, 1989·Journal of Bacteriology·M Werner-WashburneE A Craig
Dec 1, 1987·Journal of Bacteriology·T HottigerA Wiemken
Jan 1, 1974·Advances in Carbohydrate Chemistry and Biochemistry·A D Elbein
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies

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Citations

Mar 26, 2004·FEMS Yeast Research·Jaime Aguilera, Jose Antonio Prieto
Jan 12, 2001·FEMS Microbiology Reviews·J François, J L Parrou
Apr 25, 2003·Trends in Genetics : TIG·Vera van NoortMartijn A Huynen
Mar 6, 1999·Current Opinion in Microbiology·K A MoranoD J Thiele
Oct 20, 2005·Journal of Bioscience and Bioengineering·Y OgawaK Ito
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·A P Schmitt, K McEntee
Jul 31, 1998·Protein Science : a Publication of the Protein Society·M C ThallerG M Rossolini
Oct 23, 2002·Molecular and Cellular Biology·Matt KaeberleinLeonard Guarente
Jan 20, 2006·The Journal of Parasitology·Ganpati B JagdaleSeppo O Salminen
Jul 18, 2002·Molecular Plant-microbe Interactions : MPMI·Avid BrodmannAstrid Wingler
Sep 19, 2003·International Journal for Parasitology·F I PelleroneA C Somerville
Mar 4, 2008·Biological & Pharmaceutical Bulletin·Yingying CaoGuoQing Zhang
Oct 6, 1998·Annual Review of Biochemistry·R C Dickson
Dec 3, 2013·Molecular Biology Reports·Yunyan DengDelin Duan
Sep 8, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·J AchillesW Babel
Jan 9, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Waruenada KanprasoetAmornrat Naranuntarat Jensen
Feb 18, 2010·Journal of Bioscience and Bioengineering·Siraje Arif MahmudHiroshi Shimizu
May 17, 2007·The New Phytologist·Aurora OcónNatalia Requena

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