Disruption of Vi bacteriophage III and localization of its deacetylase activity

The Journal of General Virology
B KwiatkowskiS Stirm

Abstract

It has been shown that particles of Vi bacteriophage III catalyse deacetylation of O-acetyl pectic (polygalacturonic) acid, a structural analogue of Vi polysaccharide (Vi antigen). Using this substrate, and determining the acetic acid liberated by gas-liquid chromatogrphy, a method for the estimation of Vi phage deacetylase activity has been developed. Purified particles of Vi phage III were exposed to a variety of mildly dissociative reagents and conditions, and then tested for plaque-forming and for deacetylase activity. They have also been inspected under the electron microscope. Osmotic shock, and incubation in the presence of ethylenediamine tetraacetic acid (greater than or equil 0-01 M), or of L-arginine (0-25 M), were found to cause disintegration of the virions into empty head capsids, deoxyribonucleic acid, and base plates still carrying the spikes. The mixtures of viral fragments exhibited an increased deacetylase activity. Using zonal sedimentation and ion exchange chromatography, the phage fragments obtained by treatment with ethylenediaminetetraacetic acid have been fractionated and the base plates isolated. Amongst the viral components, these structures showed the highest specific deacetylase activity. They had t...Continue Reading

Citations

Oct 1, 1984·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·K B Jastrzemski, B Kwiatkowski
Dec 14, 1979·Biochemical and Biophysical Research Communications·B Kwiatkowski, K B Jastrzemski
May 18, 2017·Molecular Microbiology·Nikolai S ProkhorovAndrey V Letarov

Related Concepts

Acetic Acids
Acetylesterase
Bacteriophages
Citrobacter
Edetic Acid, Sodium Salt
Paracolobactrum
Exoglycosidases
Hydrogen-Ion Concentration
Osmosis
Polypeptides

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