Mar 4, 2014

Dissecting a known RNA-protein interaction using a yeast three-hybrid system

Methods in Enzymology
Yvonne Y Koh, Marvin Wickens

Abstract

The yeast three-hybrid system has been applied to known protein-RNA interactions for a variety of purposes. For instance, protein and RNA mutants with altered or relaxed binding specificities can be identified. Mutant RNAs can also be analyzed to better understand RNA-binding specificity of a specific protein. Furthermore, this system complements other biochemical techniques, for example, SELEX, co-immunoprecipitation and cross-linking experiments (see UV crosslinking of interacting RNA and protein in cultured cells and PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins).

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Mentioned in this Paper

Transformation, Genetic
Complement System Proteins
Antibiotics, Antifungal
Ribonucleosides
Plasma Protein Binding Capacity
RNA-Protein Interaction
Yeast Three-Hybrid System Techniques
Mutant
Antibiotic Resistance, Fungal
Amitrole

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