Dissecting alternative splicing in the formation of Miltenberger glycophorin subtype III (GYP.Mur)

Vox Sanguinis
Kate HsuT-Y Lee

Abstract

Miltenberger subtype III (Mi.III, GP.Mur) is one of the most important red cell phenotypes in the fields of transfusion in South-East Asia. GP.Mur is believed to evolve from homologous gene recombination events between glycophorin A (GYPA) and glycophorin B (GYPB). GYP.Mur differs from GYPB in only seven nucleotides dispersed near the region of 3' exon 3 of GYP.Mur. The goal of this study was to dissect how these nucleotide variants affected splicing of exon 3. We first designed two minigene constructs: one containing GYP.Mur from exon 2 to exon 4 and the other containing GYPB in the same region. To test how these nucleotide variations between GYP.Mur and GYPB affected the splicing, a repertoire of the GYP.Mur-like minigene constructs with different point mutations were created. These minigene variants were evaluated for their abilities to induce splicing of exon 3 using a heterologous expression system. (1) GYP.Mur minigene expressed exons 2, 3 and 4, whereas GYPB minigene expressed only exon 2 and exon 4. (2) The single nucleotide alteration at the position of the 5' splice site of glycophorin intron 3 reversed the splicing decision. (3) The nucleotide variations between GYP.Mur and GYPB other than that at the 5' splice site ...Continue Reading

References

Jul 1, 1992·Transfusion Medicine Reviews·P TippettD J Anstee
Dec 1, 1991·Baillière's Clinical Haematology·C H HuangO O Blumenfeld
Apr 1, 1994·Transfusion·R E Broadberry, M Lin
Jun 1, 1996·Transfusion Medicine·R E Broadberry, M Lin
Feb 14, 1998·Transfusion Medicine Reviews·M Lin, R E Broadberry
Jun 26, 2003·Nucleic Acids Research·Luca CartegniAdrian R Krainer
Jun 25, 2004·Nucleic Acids Research·William G FairbrotherChristopher B Burge
Aug 2, 2005·Molecular and Cellular Biology·Xiang H-F ZhangLawrence A Chasin
Jul 11, 2006·Human Molecular Genetics·Philip J SmithAdrian R Krainer
Jun 15, 2007·Transfusion Medicine·P PalacajornsukM E Reid
Dec 17, 2008·Biochemical and Biophysical Research Communications·Dong-Joon KimYoung-Youl Kim
Apr 3, 2009·Nucleic Acids Research·François-Olivier DesmetChristophe Béroud
Feb 25, 2011·Transfusion Medicine Reviews·Damien J HeathcoteRobert L Flower
Jun 13, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Kate HsuYung-Syu Chan

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