Dissecting the Acid Stress Response of Rhizobium tropici CIAT 899

Frontiers in Microbiology
Julio Guerrero-CastroChristian Sohlenkamp

Abstract

Rhizobium tropici CIAT899 is a nodule-forming α-proteobacterium displaying intrinsic resistance to several abiotic stress conditions such as low pH and high temperatures, which are common in tropical environments. It is a good competitor for Phaseolus vulgaris (common bean) nodule occupancy at low pH values, however little is known about the genetic and physiological basis of the tolerance to acidic conditions. To identify genes in R. tropici involved in pH stress response we combined two different approaches: (1) A Tn5 mutant library of R. tropici CIAT899 was screened and 26 acid-sensitive mutants were identified. For 17 of these mutants, the transposon insertion sites could be identified. (2) We also studied the transcriptomes of cells grown under different pH conditions using RNA-Seq. RNA was extracted from cells grown for several generations in minimal medium at 6.8 or 4.5 (adapted cells). In addition, we acid-shocked cells pre-grown at pH 6.8 for 45 min at pH 4.5. Of the 6,289 protein-coding genes annotated in the genome of R. tropici CIAT 899, 383 were differentially expressed under acidic conditions (pH 4.5) vs. control condition (pH 6.8). Three hundred and fifty one genes were induced and 32 genes were repressed; only 1...Continue Reading

References

Jul 1, 1991·International Journal of Systematic Bacteriology·E Martínez-RomeroM A Pardo
May 5, 1991·Journal of Molecular Biology·F W Studier
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Jan 4, 1969·Nature·D Zipser
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
May 14, 1999·Current Opinion in Microbiology·J W Foster
May 29, 1999·Journal of Bacteriology·M P Castanie-CornetJ W Foster
Dec 11, 1999·Nucleic Acids Research·R L TatusovE V Koonin
Apr 18, 2000·Infection and Immunity·A P Teixeira-GomesM S Zygmunt
Sep 6, 2000·Letters in Applied Microbiology·G HuangR G Birch
Jul 5, 2001·International Journal of Medical Microbiology : IJMM·J P AudiaJ W Foster
Feb 11, 2003·Molecular Plant-microbe Interactions : MPMI·Pablo VinuesaDietrich Werner
Sep 23, 2003·Infection and Immunity·Yi WenGeorge Sachs
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Jan 20, 2004·Nature Structural & Molecular Biology·Manuel DiezPeter Gräber
Feb 27, 2004·Nature·Alessio Accardi, Christopher Miller
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Michael K. Udvardi, David A. Day
Jul 30, 2004·Biochimica Et Biophysica Acta·E PadanL Galili
Oct 21, 2004·Nature Reviews. Microbiology·John W Foster
Apr 7, 2006·Applied and Environmental Microbiology·Shawn M D BearsonMark A Rasmussen
May 5, 2006·Journal of Bacteriology·Michael PflockDagmar Beier
Jul 21, 2006·Journal of Bacteriology·Jasper Kieboom, Tjakko Abee
Mar 24, 2007·Microbiology·Cecilia I MugliaO Mario Aguilar
May 1, 2007·Molecular Cell·Lynne R ProstSamuel I Miller
Dec 31, 2008·Applied and Environmental Microbiology·Andrea L Zbell, Robert J Maier
Jan 19, 2010·Trends in Microbiology·Peter W HarrisonJ Peter W Young
Apr 21, 2010·PloS One·Ken NoguchiJoan L Slonczewski

❮ Previous
Next ❯

Citations

Nov 22, 2020·FEMS Microbiology Ecology·Juliet F NilssonMariano Pistorio
Aug 23, 2019·Journal of Proteome Research·Juliet F NilssonMariano Pistorio

❮ Previous
Next ❯

Datasets Mentioned

BETA
CP004018
GSE108074

Methods Mentioned

BETA
PCR
RNA-Seq

Software Mentioned

Bowtie2
NOISeq
R
Tm calculator
samtools
Bioconductor

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.