Dissecting the polysaccharide-rich grape cell wall changes during winemaking using combined high-throughput and fractionation methods

Carbohydrate Polymers
Yu GaoJohn P Moore

Abstract

Limited information is available on grape wall-derived polymeric structure/composition and how this changes during fermentation. Commercial winemaking operations use enzymes that target the polysaccharide-rich polymers of the cell walls of grape tissues to clarify musts and extract pigments during the fermentations. In this study, we have assessed changes in polysaccharide composition/turnover throughout the winemaking process by applying recently developed cell wall profiling approaches for monosaccharide composition (GC-MS), infra-red (IR) spectroscopy and comprehensive microarray polymer profiling (CoMPP). CoMPP performed on the concentrated soluble wine polysaccharides showed a fraction rich in rhamnogalacturonan I (RGI), homogalacturonan (HG) and arabinogalactan proteins (AGPs). We also used chemical and enzymatic fractionation techniques in addition to CoMPP to understand the berry deconstruction process more in-depth. CoMPP and gravimetric analysis of the fractionated pomace used aqueous buffers and CDTA solutions to obtain a pectin-rich fraction (pulp tightly-bound to skins) containing HG, RGI and AGPs; and then alkali (sodium carbonate and potassium hydroxide), liberating a xyloglucan-rich fraction (mainly skins). Inte...Continue Reading

References

Jan 1, 1985·Journal of Cell Science. Supplement·A G DarvillP Chelf
Nov 10, 1998·Plant Physiology·K J NunanG B Fincher
Dec 25, 2004·Science·Chris SomervilleHeather Youngs
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Isabel MollerWilliam G T Willats
Sep 4, 2007·The Plant Journal : for Cell and Molecular Biology·Sandra C Sharples, Stephen C Fry
Oct 18, 2007·Journal of Agricultural and Food Chemistry·Marguerite Dols-LafargueAline Lonvaud-Funel
Nov 16, 2007·Journal of Agricultural and Food Chemistry·Zenaida Guadalupe, Belén Ayestarán
May 24, 2008·BMC Plant Biology·Susan E MarcusJ Paul Knox
Mar 24, 2010·Planta·M-C RaletF Guillon
Apr 6, 2011·Journal of Agricultural and Food Chemistry·Naiara Busse-ValverdeAna B Bautista-Ortín
Aug 7, 2012·Journal of Agricultural and Food Chemistry·Keren A BindonJames A Kennedy
Mar 1, 2006·Functional Plant Biology : FPB·David A Brummell

❮ Previous
Next ❯

Citations

Aug 27, 2015·Journal of Agricultural and Food Chemistry·Anscha J J ZietsmanMelané A Vivier
Jul 6, 2016·Frontiers in Plant Science·Lu ZhaoJoshua J Blakeslee
Jul 31, 2018·FEMS Yeast Research·Lethiwe L MbuyaneBenoit Divol
Jan 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu GaoJohn P Moore
Dec 29, 2019·Biomolecules·Andrea Osete-AlcarazEncarna Gómez-Plaza
Jun 12, 2020·International Journal of Biological Macromolecules·Cristiane ColodelCarmen Lúcia de Oliveira Petkowicz
Apr 4, 2021·Food Chemistry·Leticia Martínez LapuenteEncarna Gómez-Plaza
May 7, 2021·Critical Reviews in Food Science and Nutrition·Gonzalo Garrido-BañuelosWessel du Toit
Jun 20, 2021·Comprehensive Reviews in Food Science and Food Safety·Pangzhen ZhangZhongxiang Fang
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Leticia Martínez-LapuenteBelén Ayestarán
Nov 26, 2021·Journal of the Science of Food and Agriculture·Elissa Abi-HabibCéline Poncet-Legrand

❮ Previous
Next ❯

Related Concepts

Related Feeds