Dissecting the role of the CRMP2-neurofibromin complex on pain behaviors

Aubin MoutalRajesh Khanna


Neurofibromatosis type 1 (NF1), a genetic disorder linked to inactivating mutations or a homozygous deletion of the Nf1 gene, is characterized by tumorigenesis, cognitive dysfunction, seizures, migraine, and pain. Omic studies on human NF1 tissues identified an increase in the expression of collapsin response mediator protein 2 (CRMP2), a cytosolic protein reported to regulate the trafficking and activity of presynaptic N-type voltage-gated calcium (Cav2.2) channels. Because neurofibromin, the protein product of the Nf1 gene, binds to and inhibits CRMP2, the neurofibromin-CRMP2 signaling cascade will likely affect Ca channel activity and regulate nociceptive neurotransmission and in vivo responses to noxious stimulation. Here, we investigated the function of neurofibromin-CRMP2 interaction on Cav2.2. Mapping of >275 peptides between neurofibromin and CRMP2 identified a 15-amino acid CRMP2-derived peptide that, when fused to the tat transduction domain of HIV-1, inhibited Ca influx in dorsal root ganglion neurons. This peptide mimics the negative regulation of CRMP2 activity by neurofibromin. Neurons treated with tat-CRMP2/neurofibromin regulating peptide 1 (t-CNRP1) exhibited a decreased Cav2.2 membrane localization, and uncoup...Continue Reading


Jul 1, 1994·Journal of Neuroscience Methods·S R ChaplanT L Yaksh
Mar 1, 1996·Pain·T J BrennanG F Gebhart
Aug 15, 1998·Journal of Structural Biology·C SatoA Engel
Mar 27, 1999·Brain : a Journal of Neurology·A CréangePierre Wolkenstein
Nov 10, 2000·Current Opinion in Neurology·H Manji
Nov 16, 2001·Archives of Dermatology·Pierre WolkensteinA Leplège
Oct 31, 2002·Journal of Child Neurology·David H Gutmann
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Dec 1, 1976·Physiology & Behavior·T L Yaksh, T A Rudy
Mar 18, 2004·Journal of the American Chemical Society·I G DenisovS G Sligar
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Berkley A LynchBruno Fuks
Aug 4, 2004·Brain : a Journal of Neurology·Alain DrouetAlain Créange
Oct 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Amy B HarkinsAaron P Fox
Dec 8, 2004·Current Drug Targets. CNS and Neurological Disorders·Joseph G McGivern, Stefan I McDonough
Aug 12, 2005·Journal of Neurophysiology·Yue WangC M Hingtgen
Nov 7, 2006·Pain·Yu-Qing Cao
Jun 15, 2007·Journal of Biochemistry and Molecular Biology·Rajesh KhannaElise F Stanley
Aug 10, 2007·The European Journal of Neuroscience·Rajesh KhannaElise F Stanley
Feb 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jan HendrichAnnette C Dolphin
Mar 4, 2008·Biochemical and Biophysical Research Communications·Yi-Ling Lin, Yi-Ping Hsueh
Oct 9, 2008·The Journal of Pharmacology and Experimental Therapeutics·Alejandra M PacchioniPeter W Kalivas
Nov 12, 2009·Methods in Enzymology·T K RitchieS G Sligar


Apr 19, 2018·Expert Opinion on Therapeutic Targets·James A Walker, M Upadhyaya
Aug 25, 2017·Channels·Aubin MoutalRajesh Khanna
Mar 28, 2019·Molecular Neurobiology·Aubin MoutalRajesh Khanna
Dec 20, 2018·Molecular Neurobiology·Jie YuRajesh Khanna
Oct 27, 2018·Neuronal Signaling·Lindsey A Chew, Rajesh Khanna
Nov 5, 2019·Channels·Aubin MoutalRajesh Khanna
Aug 11, 2020·Pain·Kimberly GomezRajesh Khanna
Apr 16, 2018·Neuroscience·Aubin MoutalRajesh Khanna

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.