Dissecting the sequential assembly and localization of intraflagellar transport particle complex B in Chlamydomonas

PloS One
Elizabeth Richey, Hongmin Qin

Abstract

Intraflagellar transport (IFT), the key mechanism for ciliogenesis, involves large protein particles moving bi-directionally along the entire ciliary length. IFT particles contain two large protein complexes, A and B, which are constructed with proteins in a core and several peripheral proteins. Prior studies have shown that in Chlamydomonas reinhardtii, IFT46, IFT52, and IFT88 directly interact with each other and are in a subcomplex of the IFT B core. However, ift46, bld1, and ift88 mutants differ in phenotype as ift46 mutants are able to form short flagella, while the other two lack flagella completely. In this study, we investigated the functional differences of these individual IFT proteins contributing to complex B assembly, stability, and basal body localization. We found that complex B is completely disrupted in bld1 mutant, indicating an essential role of IFT52 for complex B core assembly. Ift46 mutant cells are capable of assembling a relatively intact complex B, but such complex is highly unstable and prone to degradation. In contrast, in ift88 mutant cells the complex B core still assembles and remains stable, but the peripheral proteins no longer attach to the B core. Moreover, in ift88 mutant cells, while complex ...Continue Reading

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Related Concepts

IFT88 protein, Chlamydomonas
Chlamydomonas
Flagella
Microtubules
Plasma Protein Binding Capacity
Protozoan Proteins
Protein Targeting
Ciliary Body
Flagella
Chlamydomonas reinhardtii

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