Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells.

PloS One
Heidi Pertl-ObermeyerGerhard Obermeyer

Abstract

The Characeae are multicellular green algae with very close relationship to land plants. Their internodal cells have been the subject of numerous (electro-)physiological studies. When exposed to light, internodal cells display alternating bands of low and high pH along their surface in order to facilitate carbon uptake required for photosynthesis. Here we investigated for the first time the subcellular membrane protein composition of acidic and alkaline regions in internodal cells of Chara australis R. Br. using MS-proteomics. The identified peptides were annotated to Chara unigenes using a custom-made Chara database generated from a transcriptome analysis and to orthologous Arabidopsis genes using TAIR (The Arabidopsis Information Resource) database. Apart from providing the first public-available, functionally-annotated sequence database for Chara australis, the proteome study, which is supported by immunodetection, identified several membrane proteins associated with acidic regions that contain a high density of specific plasma membrane (PM) invaginations, the charasomes, which locally increase the membrane area to overcome diffusion limitation in membrane transport. An increased abundance of PM H+ ATPases at charasomes is c...Continue Reading

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Mar 5, 2019·Biophysics Reviews·M J Beilby
Jul 11, 2020·Biology of the Cell·Ilse FoissnerAlexander A Bulychev
Aug 2, 2019·Protoplasma·Alexey EreminIlse Foissner
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May 4, 2021·Journal of Extracellular Vesicles·Giorgia AdamoAntonella Bongiovanni

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Datasets Mentioned

BETA
PXD006785

Methods Mentioned

BETA
RNAseq
electrophoresis
dissection

Software Mentioned

blastn
BioNJ
InterProScan
Andromeda
cRACKER
BLAST
MapMan BINs
META7
MaxQuant
MapMan

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