Dissecting the transcriptional networks underlying breast cancer: NR4A1 reduces the migration of normal and breast cancer cell lines.

Breast Cancer Research : BCR
Annika N AlexopoulouParmjit S Jat

Abstract

Breast cancer currently accounts for more than one-quarter of all female cancers and, despite the great progress in treatment observed in the past few years, the need for identification of new gene targets that can be used for diagnosis, prognosis and therapy is evident. A previous study identified the transcription factor NR4A1 as a gene upregulated in primary breast cancer compared with normal tissue by microarray analysis and sequencing technologies. The purpose of the study was to identify the role of NR4A1 in normal mammary epithelial and breast cancer cell biology. NR4A1 expression in breast tumours was assessed by semiquantitative and real-time PCR using RNA from normal and tumour samples or breast cancer cell lines. Immunohistochemistry on tissue microarrays was performed to check NR4A1 protein expression in breast tumours. MCF-10A and 226L normal mammary epithelial cells as well as the tumour lines PMC42, ZR-75-1 and MDA-MB-231 were transduced with full-length NR4A1, and the ability of NR4A1-overexpressing cells to migrate was tested using scratch wound or transwell migration assays. Proliferation was measured using the MTT and BrdU assays, while apoptosis was determined by the Annexin V assay. The ability of the cells...Continue Reading

References

Aug 15, 1987·International Journal of Cancer. Journal International Du Cancer·H J ThorneR H Whitehead
Sep 1, 1994·Brain Research. Molecular Brain Research·J HonkaniemiM Pelto-Huikko
Oct 7, 1997·Molecular and Cellular Biology·A PhilipsJ Drouin
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·M J PageM J O'Hare
Nov 24, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J SmalleyM J O'Hare
Aug 30, 2000·Nature·C M PerouD Botstein
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J O'HareP S Jat
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SørlieA L Børresen-Dale
Feb 2, 2002·Nature·Laura J van 't VeerStephen H Friend
Dec 7, 2002·Nature Genetics·Sridhar RamaswamyTodd R Golub
Nov 1, 2003·The International Journal of Biochemistry & Cell Biology·Xiao feng YeWen jin Su
Nov 13, 2003·Molecular and Cellular Biology·Siva Kumar KolluriXiao-kun Zhang
Sep 24, 2004·The Journal of Biological Chemistry·Young-Gun YooMi-Ock Lee
Jan 25, 2005·Cardiovascular Research·José Martínez-González, Lina Badimon
Jul 12, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jong B KimMike J O'Hare
Aug 2, 2005·Nature Reviews. Cancer·Britta WeigeltLaura J van 't Veer
Dec 21, 2005·The Journal of Cell Biology·Hanna Y IrieJoan S Brugge
Mar 8, 2006·The Journal of Experimental Medicine·Huiyan ZengHarold F Dvorak
Apr 11, 2006·Nuclear Receptor Signaling·Megan A Maxwell, George E O Muscat
May 26, 2006·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Qin JiangYinsheng Wan
Jul 29, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Peter I BontaCarlie J M de Vries
Nov 14, 2006·The International Journal of Biochemistry & Cell Biology·Annika N AlexopoulouJohn R Couchman
Nov 24, 2006·Investigative Ophthalmology & Visual Science·Zheng WangPeter S Reinach
Dec 1, 2006·Trends in Neurosciences·Daniel Lévesque, Claude Rouillard
Feb 14, 2007·Cancer Biology & Therapy·Hong YuXiaowei Xu
May 15, 2007·Journal of Clinical Pathology·L Da SilvaS R Lakhani
May 23, 2007·Nature Medicine·Shannon E MullicanOrla M Conneely
Sep 22, 2007·Current Opinion in Lipidology·Thijs W H PolsCarlie J M de Vries
Apr 24, 2008·Journal of Molecular and Cellular Cardiology·Stephen J FullerPeter H Sugden
May 20, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Emad A RakhaIan O Ellis
Jul 1, 2008·Breast Cancer Research : BCR·Anna GitCarlos Caldas
Nov 26, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Min Hu, Kornelia Polyak
Feb 21, 2009·The Biochemical Journal·Charles H Streuli, Nasreen Akhtar
Jul 22, 2009·Applied Immunohistochemistry & Molecular Morphology : AIMM·Enrique LermaJaime Prat
Aug 5, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anita GrigoriadisA Munro Neville

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Citations

Jul 25, 2012·AJR. American Journal of Roentgenology·Thomas H Berquist
Nov 21, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Siva Sai Krishna DasaLiaohai Chen
Jan 6, 2011·Expert Opinion on Therapeutic Targets·Syng-Ook LeeStephen Safe
Mar 6, 2012·Immunology·Susan LapthorneFergus Shanahan
Sep 5, 2015·Metallomics : Integrated Biometal Science·Alberta BergamoGianni Sava
Aug 1, 2015·Endocrine-related Cancer·Erik HedrickStephen Safe
Apr 28, 2012·Expert Opinion on Therapeutic Targets·Sally K Y ToAlice S T Wong
Oct 1, 2014·PloS One·Glenda EgerCarl-Henrik Heldin
Jun 3, 2016·Breast Cancer Research and Treatment·Joseph GeradtsGenglin Jin
Feb 4, 2016·PloS One·Alexa TengaTaosheng Chen
Jan 10, 2018·Frontiers in Molecular Neuroscience·Michael P GreenwoodDavid Murphy
Dec 4, 2013·Molecular Endocrinology·Stephen SafeSyng-Ook Lee
Jan 15, 2017·Journal of Molecular Endocrinology·Tram B DoanChristine L Clarke
May 3, 2019·Journal of Cellular and Molecular Medicine·Linying SunYueran Zhao
May 24, 2019·Breast Cancer Research and Treatment·Erik HedrickStephen Safe
May 9, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Helen M MohanEvelyn P Murphy
May 31, 2013·Cancer Research·Andrew J WilsonDineo Khabele
Oct 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Peng-Bo YangQiao Wu

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Methods Mentioned

BETA
PCR
FCS
flow cytometry

Software Mentioned

Excel
Improvision Volocity
SPSS
ImageJ
AxioVision
FloJo

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