Dissection of combinatorial control by the Met4 transcriptional complex.

Molecular Biology of the Cell
Traci LeeMike Tyers

Abstract

Met4 is the transcriptional activator of the sulfur metabolic network in Saccharomyces cerevisiae. Lacking DNA-binding ability, Met4 must interact with proteins called Met4 cofactors to target promoters for transcription. Two types of DNA-binding cofactors (Cbf1 and Met31/Met32) recruit Met4 to promoters and one cofactor (Met28) stabilizes the DNA-bound Met4 complexes. To dissect this combinatorial system, we systematically deleted each category of cofactor(s) and analyzed Met4-activated transcription on a genome-wide scale. We defined a core regulon for Met4, consisting of 45 target genes. Deletion of both Met31 and Met32 eliminated activation of the core regulon, whereas loss of Met28 or Cbf1 interfered with only a subset of targets that map to distinct sectors of the sulfur metabolic network. These transcriptional dependencies roughly correlated with the presence of Cbf1 promoter motifs. Quantitative analysis of in vivo promoter binding properties indicated varying levels of cooperativity and interdependency exists between members of this combinatorial system. Cbf1 was the only cofactor to remain fully bound to target promoters under all conditions, whereas other factors exhibited different degrees of regulated binding in a ...Continue Reading

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Citations

Jul 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Allegra A PettiDavid Botstein
Dec 16, 2011·The Journal of Biological Chemistry·Peggy Baudouin-CornuJean Labarre
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Datasets Mentioned

BETA
E-MEXP-2427
E-MEXP-2429

Methods Mentioned

BETA
immunoprecipitation
PCR
ChIP

Software Mentioned

MAST
MEME

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