Dissection of hippocampal dentate gyrus from adult mouse.

Journal of Visualized Experiments : JoVE
Hideo HagiharaTsuyoshi Miyakawa

Abstract

The hippocampus is one of the most widely studied areas in the brain because of its important functional role in memory processing and learning, its remarkable neuronal cell plasticity, and its involvement in epilepsy, neurodegenerative diseases, and psychiatric disorders. The hippocampus is composed of distinct regions; the dentate gyrus, which comprises mainly granule neurons, and Ammon's horn, which comprises mainly pyramidal neurons, and the two regions are connected by both anatomic and functional circuits. Many different mRNAs and proteins are selectively expressed in the dentate gyrus, and the dentate gyrus is a site of adult neurogenesis; that is, new neurons are continually generated in the adult dentate gyrus. To investigate mRNA and protein expression specific to the dentate gyrus, laser capture microdissection is often used. This method has some limitations, however, such as the need for special apparatuses and complicated handling procedures. In this video-recorded protocol, we demonstrate a dissection technique for removing the dentate gyrus from adult mouse under a stereomicroscope. Dentate gyrus samples prepared using this technique are suitable for any assay, including transcriptomic, proteomic, and cell biolog...Continue Reading

References

Oct 29, 1998·Neuroscience Letters·A M InsaustiT F Vallina
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey M RedwineFloyd E Bloom
Apr 16, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ed S LeinFred H Gage

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Citations

Feb 8, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Keizo TakaoTsuyoshi Miyakawa
Mar 18, 2011·Molecular Brain·Katsunori KobayashiHidenori Suzuki
Jul 31, 2012·The Journal of Nutritional Biochemistry·Anna R PattenBrian R Christie
Oct 10, 2015·Stem Cell Research·Deqiang LiJonathan A Epstein
Sep 29, 2011·The EMBO Journal·Athanasios ZovoilisAndre Fischer
Aug 30, 2011·The EMBO Journal·Roberto Carlos Agis-BalboaAndre Fischer
Feb 27, 2013·The European Journal of Neuroscience·Heather D VanGuilder StarkeyWillard M Freeman
Mar 31, 2016·Cell Reports·Hideo HagiharaTsuyoshi Miyakawa
Apr 7, 2016·Stem Cell Reports·Tara L WalkerGerd Kempermann
Jul 11, 2013·Neural Plasticity·Hideo HagiharaTsuyoshi Miyakawa
Sep 5, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Atsushi ToyodaTakamitsu Tsukahara
May 15, 2016·Journal of Neuroinflammation·Kristen N FantettiLauren A O'Donnell
Aug 10, 2016·Molecular and Cellular Neurosciences·K Sánchez-HuertaJ Pacheco-Rosado

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Datasets Mentioned

BETA
AI098840

Methods Mentioned

BETA
dissect
dissection
PCR

Software Mentioned

Microarray Analysis Suite

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