Dissection of human tropoelastin: solution structure, dynamics and self-assembly of the exon 5 peptide

Chemistry : a European Journal
Brigida BochicchioAntonio M Tamburro

Abstract

The elastic properties of elastin have essentially been discussed in terms of dominant entropic components, with questions still remaining about whether the basic mechanism is compatible with the classical theory of rubber elasticity. A better understanding of the structure-function relationships in terms of the protein's elastic properties remains an important goal in elastin science. Recently, we succeeded in the exon-by-exon synthesis of all polypeptide sequences encoded by the so-called hydrophobic exons and almost all of the cross-linking exons of human tropoelastin. Among these, the peptide encoded by exon 5 (PGGLAGAGLGA) has been extensively studied by classical spectroscopic methods, such as CD and NMR spectroscopy, and by molecular dynamics simulations. The results obtained clearly evidenced a large flexibility of the polypeptide chain, which oscillates between rather extended conformations, such as PPII, and folded ones, such as beta turns. At the supramolecular level, we obtained evidence by TEM that shows that the peptide encoded by exon 5 is able to self-assemble in fibrillar structures, a result indicating that the "information" for self-assembly is also contained within a small domain of tropoelastin.

Citations

Jul 1, 2005·Chirality·Brigida BochicchioAntonio M Tamburro
Jun 18, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Nicolas FloquetAlain J P Alix
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lisa D MuiznieksFred W Keeley
Aug 25, 2007·Nanomedicine·Antonietta PepeAntonio M Tamburro
Apr 7, 2005·Biopolymers·Gautier MoroyStéphanie Héry-Huynh
Feb 23, 2008·Chirality·Brigida BochicchioAntonio M Tamburro
Dec 3, 2011·Chirality·Brigida Bochicchio, Antonietta Pepe
Jul 1, 2008·Biophysical Journal·Kate M NairnChristopher M Elvin
Aug 19, 2007·Biophysical Journal·Angela OstuniAntonio M Tamburro
May 14, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Antonietta PepeAntonio M Tamburro
Mar 27, 2015·Soft Matter·Brigida BochicchioManuel Dauchez
Jun 11, 2005·Macromolecular Bioscience·Brigida BochicchioAntonio M Tamburro
Nov 20, 2004·The Journal of Biological Chemistry·Antonio Mario TamburroIvonne Pasquali Ronchetti
Mar 16, 2021·Frontiers in Bioengineering and Biotechnology·Jazmin OzsvarAnthony S Weiss
Jun 15, 2007·Biochemistry·Lisa D Muiznieks, Anthony S Weiss
Aug 2, 2006·Biochemistry·Antonio Mario TamburroBrigida Bochicchio
May 11, 2006·Chemical Reviews·Zhengshuang ShiNeville R Kallenbach

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