Dissection of keratin network formation, turnover and reorganization in living murine embryos

Scientific Reports
Nicole SchwarzRudolf E Leube

Abstract

Epithelial functions are fundamentally determined by cytoskeletal keratin network organization. However, our understanding of keratin network plasticity is only based on analyses of cultured cells overexpressing fluorescently tagged keratins. In order to learn how keratin network organization is affected by various signals in functional epithelial tissues in vivo, we generated a knock-in mouse that produces fluorescence-tagged keratin 8. Homozygous keratin 8-YFP knock-in mice develop normally and show the expected expression of the fluorescent keratin network both in fixed and in vital tissues. In developing embryos, we observe for the first time de novo keratin network biogenesis in close proximity to desmosomal adhesion sites, keratin turnover in interphase cells and keratin rearrangements in dividing cells at subcellular resolution during formation of the first epithelial tissue. This mouse model will help to further dissect keratin network dynamics in its native tissue context during physiological and also pathological events.

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Citations

Jan 15, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Amélie RobertVladimir I Gelfand
Mar 21, 2016·Kidney International·Natasha T Snider
Jun 30, 2016·Cells·Florian Geisler, Rudolf E Leube
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Jan 19, 2021·Reproduction in Domestic Animals = Zuchthygiene·Deisy J D SanchezArlindo A Moura
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Dec 21, 2021·Cellular and Molecular Gastroenterology and Hepatology·Annika GrossPavel Strnad

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Methods Mentioned

BETA
environmental stress
PCR
fluorescence microscopy
transgenic
fluorescence recovery after photobleaching
light sheet microscopy
a
X-ray
genotyping
biopsies

Software Mentioned

GraphPad
AMIRA
Fiji
Prism
Excel
Zen

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