Dissection of the bridging pattern of bovicin HJ50, a lantibiotic containing a characteristic disulfide bridge

Microbiological Research
Yuheng LinJin Zhong

Abstract

Bovicin HJ50, a lantibiotic produced by Streptococcus bovis HJ50, is featured by the presence of a disulfide bridge. This study described a simplified in vitro synthetic strategy for producing bovicin HJ50 totally based on Escherichia coli expression system. In this strategy termed as Semi-in vitro biosynthesis (SIVB), prepeptide BovA and modification enzyme BovM were co-expressed to generate posttranslationally modified BovA. Then a specific protease BovT150 was employed to remove the leader peptide in vitro and produce biologically active bovicin HJ50. Via SIVB, a series of ring-broken bovicin mutants C13A, C21A, C29A and T10A/C32A were prepared by introducing site-directed mutations into bovA gene. Further, we analyzed the bridging patterns of these mutants through chemical modification and successfully clarified the bridging pattern of bovicin HJ50. The results showed that two thioether bridges exist between Thr8 and Cys13, and Thr10 and Cys32, respectively, and that the disulfide bond bridging Cys21 and 29 is very relevant for the antimicrobial activity of bovicin HJ50. This is the first study that reports the bridging pattern of bovicin HJ50.

References

Feb 1, 1996·Antonie van Leeuwenhoek·O P KuipersM J Gasson
Apr 24, 1999·European Journal of Biochemistry·A Karakas SenM J Gasson
Jan 29, 2000·European Journal of Biochemistry·C van KraaijO P Kuipers
May 24, 2000·The Journal of Biological Chemistry·R E KrullP W Caufield
Mar 2, 2002·The Journal of Biological Chemistry·Ronald DorenbosJan Maarten Van Dijl
Jul 4, 2003·Applied and Environmental Microbiology·Christiane SzekatGabriele Bierbaum
Jan 2, 2004·Microbiology·Haijie XiaoLiandong Huan
Jan 31, 2004·Science·Lili XieWilfred A van der Donk
Dec 22, 2004·Biotechnology Advances·Grzegorz Bulaj
Sep 7, 2005·Biochemical and Biophysical Research Communications·Jun-Ichi NagaoKenji Sonomoto
Apr 5, 2007·Nature Protocols·Hermann Schägger
Jun 24, 2008·Biochemistry·L A Furgerson IhnkenWilfred A van der Donk
Feb 5, 2009·Journal of Applied Microbiology·T KabukiH Nakajima

❮ Previous
Next ❯

Citations

Jun 25, 2013·Journal of the American Chemical Society·Noah A Bindman, Wilfred A van der Donk
Sep 12, 2013·The Journal of Antibiotics·Christopher T Lohans, John C Vederas
Oct 17, 2013·Biotechnology Letters·Quinn Lu, Juan C Aon
Mar 13, 2012·Annual Review of Biochemistry·Patrick J Knerr, Wilfred A van der Donk
Nov 6, 2012·Food Microbiology·Marina GeorgalakiEffie Tsakalidou
Jul 8, 2014·Biochemical and Biophysical Research Communications·Hongchu MaJin Zhong
May 2, 2016·Journal of Dairy Science·Xiaoxuan GeJin Zhong
Sep 4, 2016·FEMS Microbiology Reviews·Manuel Montalbán-LópezOscar P Kuipers
Jan 31, 2017·Chemical Reviews·Lindsay M RepkaWilfred A van der Donk
Aug 8, 2018·FEMS Microbiology Reviews·Jeella Z AcedoMarco J van Belkum
Nov 20, 2016·Applied and Environmental Microbiology·Liujie HuoWilfred A van der Donk
Feb 18, 2014·Applied and Environmental Microbiology·Jian WangJin Zhong
Dec 3, 2017·Expert Opinion on Drug Discovery·Mengxin Geng, Leif Smith
Aug 11, 2018·Frontiers in Microbiology·Beatriz Mesa-PereiraR Paul Ross
Sep 17, 2020·Natural Product Reports·Manuel Montalbán-LópezWilfred A van der Donk
Jan 19, 2021·Microbial Biotechnology·Zhiyong WuGuiyang Shi
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yanlong CuiXin Lü
Apr 7, 2012·Journal of the American Chemical Society·Trent J OmanWilfred A van der Donk
Sep 11, 2014·Journal of the American Chemical Society·Christopher T LohansJohn C Vederas
May 27, 2015·Journal of the American Chemical Society·Noah A BindmanWilfred A van der Donk
Jul 21, 2011·Journal of the American Chemical Society·Ayşe ÖkesliWilfred A van der Donk
Sep 6, 2018·Journal of the American Chemical Society·Tong SiHuimin Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Journal of the American Chemical Society
Yanxiang ShiWilfred A van der Donk
Proceedings of the National Academy of Sciences of the United States of America
Amanda L McClerrenWilfred A van der Donk
© 2021 Meta ULC. All rights reserved