Dissection of the Candida albicans Cdc4 protein reveals the involvement of domains in morphogenesis and cell flocculation

Journal of Biomedical Science
Chuen ChinJia-Ching Shieh

Abstract

CDC4, which encodes an F-box protein that is a member of the Skp1-Cdc53/Cul1-F-box (SCF) ubiquitin E3 ligase, was initially identified in the budding yeast Saccharomyces cerevisiae as an essential gene for progression through G1-S transition of the cell cycle. Although Candida albicans CDC4 (CaCDC4) can release the mitotic defect caused by the loss of CDC4 in S. cerevisiae, CaCDC4 is nonessential and suppresses filamentation. To further elucidate the function of CaCDC4, a C. albicans strain, with one CaCDC4 allele deleted and the other under the repressible C. albicans MET3 promoter (CaMET3p) control, was made before introducing cassettes capable of doxycycline (Dox)-induced expression of various C. albicans Cdc4 (CaCdc4) domains. Cells from each strain could express a specific CaCdc4 domain under Dox-induced, but CaMET3-CaCDC4 repressed conditions. Cells expressing domains without either the F-box or WD40-repeat exhibited filamentation and flocculation similarly to those lacking CaCDC4 expression, indicating the functional essentiality of the F-box and WD40-repeat. Notably, cells expressing the N-terminal 85-amino acid truncated CaCdc4 partially reverse the filament-to-yeast and weaken the ability to flocculate compared to tho...Continue Reading

References

Sep 1, 1991·Infection and Immunity·D R Kirsch, R R Whitney
Mar 22, 1996·Cell·M Hochstrasser
Sep 23, 1997·Cell·H J LoG R Fink
Nov 24, 1999·Molecular Microbiology·R S CareP E Sudbery
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A R WillemsM Tyers
Dec 4, 2001·FEMS Microbiology Letters·J M BainN A Gow
Dec 4, 2001·Current Opinion in Microbiology·H Liu
Mar 4, 2003·Current Genetics·Andrea Walther, Jürgen Wendland
Apr 9, 2005·Molecular Biology of the Cell·Avigail Atir-LandeDaniel Kornitzer
Jul 27, 2005·FEMS Yeast Research·Jennifer C BaylyAnne M Dranginis
Aug 10, 2005·Eukaryotic Cell·Yang-Nim Park, Joachim Morschhäuser
Oct 18, 2005·Journal of Biomedical Science·Jia-Ching ShiehJohn Rosamond
Mar 25, 2006·Molecular Microbiology·Kevin J Verstrepen, Frans M Klis
Oct 24, 2006·Current Opinion in Microbiology·Judith Berman
May 18, 2007·Annual Review of Microbiology·Malcolm Whiteway, Catherine Bachewich
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Subhrajit BiswasAsis Datta
Apr 7, 2010·Biochemical and Biophysical Research Communications·Tzu-Ling TsengJia-Ching Shieh
Dec 30, 2010·Nature Reviews. Microbiology·Jonathan S Finkel, Aaron P Mitchell

❮ Previous
Next ❯

Citations

Feb 27, 2015·Canadian Journal of Microbiology·Tzu-Ling TsengJia-Ching Shieh
Aug 3, 2018·The Protein Journal·Buddhi Prakash Jain, Shweta Pandey
Dec 20, 2017·Virulence·Chi-Jan LinYing-Lien Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
affinity-purification
PCR

Software Mentioned

ImageGauge
L Process
- blaster
Ura
mini

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.