PMID: 6403555Mar 1, 1983Paper

Dissection of the Golgi complex. II. Density separation of specific Golgi functions in virally infected cells treated with monensin

The Journal of Cell Biology
P QuinnG Warren

Abstract

In the accompanying paper (Griffiths, G., P. Quinn, and G. Warren, 1983, J. Cell Biol., 96:835-850), we suggested that the Golgi stack could be divided into functionally distinct cis, medial, and trans compartments, each comprising one or two adjacent cisternae. These compartments were identified using Baby hamster kidney (BHK) cells infected with Semliki Forest virus (SFV) and treated with monensin. This drug blocked intracellular transport but not synthesis of the viral membrane proteins that were shown to accumulate in the medial cisternae. In consequence, these cisternae bound nucleocapsids. Here we show that this binding markedly increased the density of the medial cisternae and allowed us to separate them from cis and trans Golgi cisternae. A number of criteria were used to show that the intracellular capsid-binding membranes (ICBMs) observed in vivo were the same as those membranes sedimenting to a higher density in sucrose gradients in vitro, and this separation of cisternae was then used to investigate the distribution, within the Golgi stack, of some specific Golgi functions. After labeling for 2.5 min with [3H]palmitate, most of the fatty acid attached to viral membrane proteins was found in the ICBM fraction. Becaus...Continue Reading

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M G RothR W Compans
Oct 1, 1969·The Journal of Cell Biology·B FleischerH Ozawa
Oct 1, 1973·The Journal of Cell Biology·J J BergeronG E Palade
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W G DunphyJ E Rothman
Jan 1, 1981·Annual Review of Biochemistry·S C Hubbard, R J Ivatt
Dec 1, 1981·The Journal of Cell Biology·M G Farquhar, G E Palade
Nov 15, 1981·Journal of Molecular Biology·J GreenG Warren
Sep 11, 1981·Science·J E Rothman
Jan 1, 1980·The Journal of Cell Biology·R BretzG E Palade

❮ Previous
Next ❯

Citations

Aug 1, 1995·Molecular and Cellular Biochemistry·R V RajuR K Sharma
Jan 1, 1986·Archives of Virology·T KohamaA Sugiura
Jan 1, 1985·The Journal of Membrane Biology·H FeracciS Maroux
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A Grossman
Oct 1, 1988·Journal of Ultrastructure and Molecular Structure Research·C Lopez-Iglesias, F Puvion-Dutilleul
Dec 21, 2002·Pharmacology & Therapeutics·Riad Qanbar, Michel Bouvier
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D Towler, L Glaser
Dec 1, 1986·The Journal of Cell Biology·G L FirestoneK R Yamamoto
Mar 1, 1988·The Journal of Cell Biology·R G Anderson, L Orci
Jun 1, 1984·The Journal of Cell Biology·H J GeuzeK Von Figura
Jul 19, 2013·Journal of Virology·Onyinyechukwu UchimeMargaret Kielian
Apr 1, 1992·Journal of Neurochemistry·R G Farrer, J A Benjamins
Jan 1, 1994·Platelets·E M Huang
Dec 27, 1993·FEBS Letters·M Veit, M F Schmidt
Jan 1, 1986·CRC Critical Reviews in Biochemistry·G J Strous
Feb 1, 1991·Journal of Electron Microscopy Technique·D M Morré
Nov 1, 1989·Experimental Cell Research·G MigliaccioS Bonatti
Aug 1, 1988·Journal of Cellular Biochemistry·A I Magee, K Siddle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.