PMID: 8593958Dec 1, 1995Paper

Dissection of the Yersinia enterocolitica virulence plasmid pYVO8 into an operating unit and virulence gene modules

FEMS Microbiology Letters
A RoggenkampJ Heesemann

Abstract

The structural genes encoding the Yop proteins of Yersinia enterocolitica are scattered around on the virulence plasmid (pYV). The genes which are required for transactivation, secretion and translocation of the Yopos are encoded in one cluster known as the lcr-region of pYV. After the introduction of an additional SalI restriction site into pYV of Y. enterocolitica serotype O8, we were able to clone and isolate the whole lcr-region on the mobilizable low copy vector pSUP102. Analysis of this construct in a plasmidless WA-strain showed that all Yops being encoded inside the lcr-region (YopN, YopB, YopD and the V-antigen) were secreted into the culture supernatant. Moreover, this lcr-fragment was able to promote secretion of other Yops encoded by a second recombinant plasmid. Thus the translocation and function of single Yops can be studied.

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Citations

Mar 11, 2004·Cellular Microbiology·Reinhard HoffmannJürgen Heesemann
Jul 19, 2003·International Journal of Medical Microbiology : IJMM·Konrad TrülzschJürgen Heesemann
Jul 29, 2006·FEMS Immunology and Medical Microbiology·Hristo NajdenskiMikael Skurnik
Jun 7, 2003·The Journal of Biological Chemistry·Martin AepfelbacherJurgen Heesemann
Nov 13, 1998·Journal of Applied Microbiology·C H MartinsD P Falcão
Aug 22, 2007·Memórias do Instituto Oswaldo Cruz·Carlos Henrique Gomes MartinsDeise Pasetto Falcão
Oct 30, 2001·FEMS Microbiology Letters·K Brzostek, A Raczkowska
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K RuckdeschelJ Heesemann

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