Dissemination of multidrug-resistant blaCTX-M-15/IncFIIk plasmids in Klebsiella pneumoniae isolates from hospital- and community-acquired human infections in Tunisia

Diagnostic Microbiology and Infectious Disease
Wejdène MansourJean-Yves Madec

Abstract

This study investigated the molecular features of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae from hospital- and community-acquired (HA/CA) infections in the region of Mahdia, Tunisia. Among 336 K. pneumoniae isolates recovered from both clinical contexts between July 2009 and December 2011, 49 and 15 were ESBL producers and originated from clinical and community sources, respectively. All isolates produced the CTX-M-15 enzyme. As shown by Southern blot on S1 nuclease treatment followed by pulsed-field gel electrophoresis (PFGE) gels, the blaCTX-M-15 gene was carried on IncFII (n=4), IncFIIk (n=25), IncL/M (n=4), IncK (n=1), or untypeable (n=15) plasmids in HA isolates. In CA isolates, the blaCTX-M-15 gene was carried on IncFIIk (n=6), IncFII (n=1), IncHI1 (n=1), or untypeable (n=7) plasmids. In all, 23 and 11 PFGE types were found among the HA and CA isolates. Multilocus sequence typing on representative isolates shows diverse sequence types (STs), such as ST307, ST101, ST39, ST4, ST140, ST15, and ST307 in HA isolates and ST101, ST664, and ST323 in CA isolates. This study is the first comprehensive report of ESBL plasmids in K. pneumoniae from HA and CA infections in Tunisia.

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Citations

Jun 14, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ghassan TayhKarim Ben Slama
Jul 18, 2017·Journal of Global Antimicrobial Resistance·Wejdène MansourJean-Yves Madec
Jan 18, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Claire L GorrieKathryn E Holt
Dec 13, 2017·Antimicrobial Agents and Chemotherapy·Nadia JaidaneThierry Naas
Oct 27, 2017·Journal of Medical Microbiology·Eva BrhelovaMartina Lengerova
Mar 11, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Nadezhda K FursovaNikolay V Volozhantsev
Aug 5, 2020·Antimicrobial Agents and Chemotherapy·Gisele PeiranoJohann D D Pitout

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