Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
B J ButowM C Gianibelli

Abstract

Increased expression of the high molecular weight glutenin subunit (HMW-GS) Bx7 is associated with improved dough strength of wheat (Triticum aestivum L.) flour. Several cultivars and landraces of widely different genetic backgrounds from around the world have now been found to contain this so-called 'over-expressing' allelic form of the Bx7 subunit encoded by Glu-B1al. Using three methods of identification, SDS-PAGE, RP-HPLC and PCR marker analysis, as well as pedigree information, we have traced the distribution and source of this allele from a Uruguayan landrace, Americano 44D, in the mid-nineteenth century. Results are supported by knowledge of the movement of wheat lines with migrants. All cultivars possessing the Glu-B1al allele can be identified by the following attributes: (1) the elution of the By sub-unit peak before the Dx sub-unit peak by RP-HPLC, (2) high expression levels of Bx7 (>39% Mol% Bx), (3) a 43 bp insertion in the matrix-attachment region (MAR) upstream of the gene promoter relative to Bx7 and an 18 bp nucleotide duplication in the coding region of the gene. Evidence is presented indicating that these 18 and 43 bp sequence insertions are not causal for the high expression levels of Bx7 as they were also f...Continue Reading

References

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Citations

Dec 20, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Catherine RavelGilles Charmet
Nov 7, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Raja RagupathySylvie Cloutier
Sep 18, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sixin LiuJames A Anderson
Nov 8, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·L H WangX C Xia
Sep 23, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xin GaoDiane E Mather
Jul 12, 2008·Molecular Biotechnology·Maria OszvaldMoon-Sik Yang
Sep 22, 2009·Genome Génome / Conseil National De Recherches Canada·O D Anderson
Oct 17, 2013·Genetics and Molecular Biology·Leonardo S VanzettiMarcelo Helguera
Jul 1, 2005·Rapid Communications in Mass Spectrometry : RCM·Gaetano AlberghinaAnna Savarino
May 11, 2020·Journal of the Science of Food and Agriculture·Ágnes BánfalviSándor Tömösközi
Dec 18, 2019·BMC Plant Biology·Silvana Marisol Luján BasileLeonardo Sebastián Vanzetti

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