Dissimilar microRNA-21 functions and targets in trophoblastic cell lines of different origin

The International Journal of Biochemistry & Cell Biology
Wittaya ChaiwangyenDiana M Morales-Prieto

Abstract

Trophoblast cells express a singular miRNA expression profile which varies during pregnancy and whose alteration may be associated with pregnancy complications. miR-21, a widely known oncomir, is highly expressed in human placenta but its role in regulating trophoblast cells remains unclear. The aim of this study was to investigate miR-21 functions and targets in HTR-8/SVneo immortalized trophoblast and JEG-3 choriocarcinoma cells, which are trophoblast cell models that differ in their cellular origin. Cells were transfected with miR-21-antagomir, -mimic or their respective controls. Following, cell proliferation (BrdU), migration (Transwell and scratch wound-healing assays), invasion (Matrigel assays) and apoptosis (flow cytometry, TUNEL assay and Western blotting) were assessed. Expression of the potential miR-21 targets phosphatase and tensin homolog (PTEN) and programmed cell death 4 (PDCD4) were analyzed by Western blotting. Inhibition of miR-21 decreased cell proliferation, migration, and invasion in JEG-3 and HTR-8/SVneo cells and additionally, induced apoptosis in JEG-3 cells. Silencing of miR-21 enhanced PDCD4 expression only in JEG-3 cells, and PTEN expression only in HTR-8/SVneo cells. Inhibition of miR-21 significan...Continue Reading

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Citations

Apr 5, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Heyam HayderChun Peng
Oct 10, 2019·Journal of Cellular Physiology·Maryam HemmatzadehMehdi Yousefi
Oct 11, 2017·Frontiers in Cardiovascular Medicine·Kelsey R BoundsPiyali Chatterjee
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May 10, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Li TangWenming Xu
May 20, 2020·International Journal of Molecular Sciences·Wittaya ChaiwangyenDiana M Morales-Prieto
Nov 27, 2020·Frontiers in Immunology·Lisvaneth MedinaUlrike Kemmerling
Apr 21, 2021·Minerva Obstetrics and Gynecology·Edward Araujo JúniorLuciano M Nardozza
Sep 1, 2021·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Kun DongZhiwei Zhang
Oct 7, 2021·European Journal of Clinical Nutrition·P KochharA Mukhopadhyay

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