Dissociated adult rat subfornical organ neurons maintain membrane properties and angiotensin responsiveness for up to 6 days

Neuroendocrinology
A V FergusonW T Mason

Abstract

We have utilised standard dissociation techniques to obtain a preparation of subfornical organ (SFO) cells that have been maintained in tissue culture for up to 1 week. Stable (> 15 min) whole cell recordings were obtained from 80 cells displaying rapid (<2 ms) voltage-dependent sodium currents (blocked by tetrodotoxin in 10 of 10 cells tested), and current evoked action potentials, which were thus classified as SFO neurons. These neurons had a resting membrane potential of-63.8 +/- 1.3 mV (mean +/- SEM), spike amplitude of 86.8 +/- 2.5 mV, and input resistance of 1.2 +/- 0.1 G omega, characteristics which did not change significantly in recordings obtained for up to 6 days after dissociation. Current clamp recording showed that of 65 cells tested with bath application of angiotensin (ANG; 1,000-10nM), 41 responded to this peptide with decreases in input resistance (control 1.4 +/- 0.16 G omega, after ANG 0.78 +/- 0.1 G omega, p < 0.0001), and depolarisations (mean 18.3 +/- 2.0 mV, p < 0.0001). Similar recordings were obtained from viable cells up to 6 days after initial cell dissociation. These studies provide the first description of the basic membrane properties of dissociated SFO neurons. The responsiveness of these cells t...Continue Reading

Citations

Apr 2, 2003·Regulatory Peptides·Bo Yang, Alastair V Ferguson
Dec 1, 2000·Neuroscience·J W AndersonA V Ferguson
Aug 3, 1999·Neuroscience·D L WashburnA V Ferguson
Jun 20, 2001·Journal of Neuroendocrinology·K OnoK Inenaga
May 21, 1999·The European Journal of Neuroscience·D L WashburnA V Ferguson
Sep 1, 2007·Neuroreport·Mark Fry, Alastair V Ferguson
Mar 6, 2013·Physiology & Behavior·Andrea MimeeAlastair V Ferguson
Dec 2, 2000·The Journal of Physiology·D L WashburnA V Ferguson
Jul 26, 2003·The Journal of Physiology·Sheana E Desson, Alastair Victor Ferguson
Aug 27, 2005·The Journal of Physiology·Kentaro OnoKiyotoshi Inenaga
Mar 13, 2014·Journal of Neuroendocrinology·M Kuksis, A V Ferguson
Dec 23, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nicole M Cancelliere, Alastair V Ferguson
Jun 5, 2018·Journal of Neuroendocrinology·A KameshA V Ferguson
Oct 27, 2018·Journal of Neuroendocrinology·Bruno Paes-LemeAlastair V Ferguson
Jul 11, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mark FryAlastair V Ferguson
Jan 17, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Al-Shaimaa F AhmedKeith A Sharkey
Feb 15, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Irving H ZuckerHanjun Wang
Jun 24, 2017·Journal of Neurophysiology·Nick J Simpson, Alastair V Ferguson
Aug 26, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Willis K SamsonAlastair V Ferguson
May 29, 2019·Journal of Neuroendocrinology·Kirthikaa BalapattabiJ Thomas Cunningham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.