Dissociated insulinotropic sensitivity to glucose and carbachol in high-fat diet-induced insulin resistance in C57BL/6J mice

Metabolism: Clinical and Experimental
B AhrénF Sundler

Abstract

To study islet function following reduced insulin sensitivity, we examined mice of the C57BL/6J strain, the genotype of which carries an increased propensity to develop insulin resistance when metabolically challenged. The mice received either a high-fat diet (58% fat on an energy basis) or a control diet (11% fat) for 12 weeks. The body weight of mice on the high-fat diet increased significantly more than that of mice on the control diet (25.8 +/- 0.4 v 21.3 +/- 0.2 g, P < .001). Already after 1 week on the high-fat diet, a significant hyperglycemia accompanied by hyperinsulinemia had evolved, indicative of insulin resistance. After 12 weeks, plasma glucose levels for high-fat diet-treated mice were 7.5 +/- 0.1 mmol/L, versus 6.5 +/- 0.1 mmol/L in controls (P < .001); corresponding values for plasma insulin were 248 +/- 17 and 104 +/- 7 pmol/L, respectively (P < .001). Mice given a high-fat diet also had elevated levels of total cholesterol, triglycerides, and free fatty acids (FFAs) compared with controls. After 4, 8, and 12 weeks, glucose (2.8, 8.3, or 16.7 mmol/kg) or the cholinergic agonist carbachol (0.16 or 0.53 micromol/kg) was injected intraperitoneally. The insulinotropic response to glucose was not different between ...Continue Reading

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Citations

Apr 25, 2003·Biochemical and Biophysical Research Communications·Maria Sörhede WinzellBo Ahrén
Aug 8, 1998·European Journal of Pharmacology·E Simonsson, B Ahrén
Apr 1, 2010·American Journal of Physiology. Endocrinology and Metabolism·Gao SunGuy A Rutter
Jul 11, 2006·The Journal of Clinical Investigation·Marc Prentki, Christopher J Nolan
Jun 2, 2000·Biochemical and Biophysical Research Communications·E SimonssonB Ahrén
Jul 26, 2011·Evidence-based Complementary and Alternative Medicine : ECAM· AnsarullahA V Ramachandran
May 15, 2015·Journal of Cellular Physiology·Sara BaldassanoFlavia Mulè
May 1, 2015·Endocrine Journal·Mototsugu NagaoShinichi Oikawa
Jun 14, 2008·Diabetes Research and Clinical Practice·Chun-Ching ShihWei-Li Lin
Aug 22, 2012·Basic & Clinical Pharmacology & Toxicology·Khalid M AlkharfyMukhtar Ahmed
Nov 16, 2004·The Journal of Nutritional Biochemistry·Misato KobayashiFumihiko Horio
Sep 11, 2004·Biochemical and Biophysical Research Communications·Maria Sörhede WinzellBo Ahrén
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Dec 11, 2013·Medical Hypotheses·Milind WatveManawa Diwekar
Jul 29, 2003·Diabetes·Maria Sörhede WinzellCecilia Holm

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