Dissociation between gene and protein expression of metabolic enzymes in a rodent model of heart failure

European Journal of Heart Failure
Eric E MorganWilliam C Stanley

Abstract

Studies in advanced heart failure show down-regulation of fatty acid oxidation genes, possibly due to decreased expression of the nuclear transcription factors peroxisome proliferator activated receptor alpha (PPARalpha) and retinoid X receptor alpha (RXRalpha). We assessed mRNA and protein expression of PPARalpha and RXRalpha, and for several PPAR/RXR regulated metabolic proteins at 8 and 20 weeks following myocardial infarction induced by coronary artery ligation. Infarction resulted in heart failure, as indicated by reduced LV fractional shortening and increased end diastolic area compared to sham. There was a progressive increase in LV end systolic area, myocardial ceramide content and atrial natriuretic peptide mRNA, and a deterioration in LV fractional area of shortening from 8 to 20 weeks. Protein and mRNA expression of PPARalpha and RXRalpha were not different among groups. The mRNA for PPAR/RXR regulated genes (e.g. medium chain acyl-CoA dehydrogenase (MCAD)) was down-regulated at 8 and 20 weeks post-infarction; however, neither the protein expression nor activity of MCAD was reduced compared to sham. In conclusion, reduced mRNA expression of PPAR/RXR regulated genes is not dependent on reduced PPAR/RXR protein express...Continue Reading

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Citations

Dec 15, 2006·Cardiovascular Research·Naveen SharmaWilliam C Stanley
Oct 16, 2008·Cardiovascular Research·Marc van BilsenGer J van der Vusse
Feb 4, 2011·Cardiovascular Research·Vincenzo LionettiFabio A Recchia
Nov 1, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Kristine M Wadosky, Monte S Willis
Aug 19, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Bruce WareVéronique A Lacombe
Mar 3, 2012·Circulation Research·William C StanleyKelly A O'Connell
Jul 30, 2010·Cardiovascular Research·Mariana G Rosca, Charles L Hoppel
Nov 26, 2010·Heart Failure Reviews·Satyam SarmaMihai Gheorghiade
Aug 26, 2009·Journal of Molecular and Cellular Cardiology·Karen M O'SheaWilliam C Stanley
Mar 3, 2009·Journal of Molecular and Cellular Cardiology·Sandra SenaSheldon E Litwin
Jun 28, 2015·Molecular and Cellular Endocrinology·Jessica E RodríguezMonte S Willis
Jul 4, 2007·Hypertension·Nathan K LebrasseurFlora Sam
Dec 13, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Eric E MorganMargaret P Chandler
Jan 21, 2010·Physiological Reviews·Gary D LopaschukWilliam C Stanley
Jun 5, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Margaret P ChandlerMartin E Young
Jun 29, 2017·Molecular Medicine Reports·Yichen GuoShu Jiang
Feb 19, 2008·Molecular and Cellular Biochemistry·Ashwin AkkiAnne-Marie L Seymour
Apr 6, 2011·Molecular and Cellular Biochemistry·Vojtech MelenovskyLudek Cervenka
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Jul 10, 2007·American Journal of Physiology. Heart and Circulatory Physiology·David J ChessWilliam C Stanley
Aug 14, 2020·Advanced Drug Delivery Reviews·T Dung Nguyen, P Christian Schulze

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